A staple‐based electrochemical platform is proposed for the first time as a simple and low‐cost detection system for paper‐based devices. The system, which incorporates small and disposable stainless‐steel staples as electrodes, is combined with a paper strip. The staple acting as the working electrode is modified with carbon ink. The platform was carefully optimized with ferrocene carboxylic acid. As a proof‐of‐concept, it was employed for the enzymatic [horseradish peroxidase (HRP)‐based] immunoelectroanalytical detection of human tissue anti‐transglutaminase (anti‐tTG), which is a biomarker for celiac disease diagnosis. The intensity of the current from the electrochemical reduction of 3,3′,5,5′‐tetramethylbenzidine (an HRP substrate) was recorded chronoamperometrically at −0.2 V in different paper areas. A linear relationship between the current measured at 30 s and the logarithm of the concentration of anti‐tTG in the range comprised between 3 and 100 U mL⁻¹ was obtained. Negative and positive controls produced expected values. Results demonstrated that the paper/staple combined platform is very convenient for the detection of electroactive analytes and other compounds that can be determined indirectly in bioassays.

中文翻译：

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基于钉书钉的纸电化学平台用于乳糜泻的诊断

首次提出了一种基于钉书钉的电化学平台，它是一种用于纸基设备的简单且低成本的检测系统。该系统将小的和一次性的不锈钢订书钉作为电极，并与纸条结合在一起。用作工作电极的订书钉用碳墨水修饰。该平台是使用二茂铁羧酸精心优化的。作为概念验证，它被用于人体组织抗转谷氨酰胺酶（anti-tTG）的酶促[基于辣根过氧化物酶（HRP）的]免疫电分析检测，这是乳糜泻诊断的生物标记。在-0.2 V下以计时电流法在不同的纸张区域记录了3,3'，5,5'-四甲基联苯胺（HRP底物）的电化学还原反应产生的电流强度。获得^-1。阴性和阳性对照均产生期望值。结果表明，纸/订书钉组合平台非常便于检测可在生物测定中间接测定的电活性分析物和其他化合物。