In recent years, bispecific antibodies (BisAbs) have emerged as novel pharmaceutical candidates owing to their ability to engage two disease mediators simultaneously, thus providing a possible alternative therapeutic approach in complex diseases such as cancer and inflammation. Here we provide an overview of the molecular design, recombinant expression in mammalian cells and purification of BisAbs based on full-length IgG-scFv formats. Practical considerations and strategies to optimize transient expression and purification are also discussed.

中文翻译：

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基于 IgG1 的双特异性二价抗体表达和纯化的分子工程策略和方法

近年来，双特异性抗体（BisAbs）由于能够同时作用于两种疾病介质而成为新型候选药物，从而为癌症和炎症等复杂疾病提供了一种可能的替代治疗方法。在这里，我们概述了分子设计、哺乳动物细胞中的重组表达以及基于全长 IgG-scFv 格式的 BisAb 纯化。还讨论了优化瞬时表达和纯化的实际考虑因素和策略。