Aphids are emerging as model organisms for both basic and applied research. Of the 5,000 estimated species, only three aphids have published whole genome sequences: the pea aphid Acyrthosiphon pisum, the Russian wheat aphid, Diuraphis noxia, and the green peach aphid, Myzus persicae. We present the whole genome sequence of a fourth aphid, the soybean aphid (Aphis glycines), which is an extreme specialist and an important invasive pest of soybean (Glycine max). The availability of genomic resources is important to establish effective and sustainable pest control, as well as to expand our understanding of aphid evolution. We generated a 302.9 Mbp draft genome assembly for Ap. glycines using a hybrid sequencing approach. This assembly shows high completeness with 19,182 predicted genes, 92% of known Ap. glycines transcripts mapping to contigs, and substantial continuity with a scaffold N₅₀ of 174,505 bp. The assembly represents 95.5% of the predicted genome size of 317.1 Mbp based on flow cytometry. Ap. glycines contains the smallest known aphid genome to date, based on updated genome sizes for 19 aphid species. The repetitive DNA content of the Ap. glycines genome assembly (81.6 Mbp or 26.94% of the 302.9 Mbp assembly) shows a reduction in the number of classified transposable elements compared to Ac. pisum, and likely contributes to the small estimated genome size. We include comparative analyses of gene families related to host-specificity (cytochrome P450's and effectors), which may be important in Ap. glycines evolution. This Ap. glycines draft genome sequence will provide a resource for the study of aphid genome evolution, their interaction with host plants, and candidate genes for novel insect control methods.

蚜虫正在成为基础和应用研究的模式生物。在估计的 5,000 个物种中，只有三种蚜虫公布了全基因组序列：豌豆蚜Acyrthosiphon pisum 、俄罗斯小麦蚜Diuraphis noxia和绿桃蚜Myzus persicae 。我们展示了第四种蚜虫——大豆蚜虫（ Aphis gigines ）的全基因组序列，它是大豆（ Glycine max ）的一种极端专家和重要的入侵害虫。基因组资源的可用性对于建立有效和可持续的害虫防治以及扩大我们对蚜虫进化的了解非常重要。我们为Ap 生成了 302.9 Mbp 的基因组组装草图。使用混合测序方法的甘氨酸。该组装显示出高度完整性，包含 19,182 个预测基因，占已知Ap 的 92%。甘氨酸转录本映射到重叠群，并且与 174,505 bp 的支架 N ₅₀具有基本连续性。该组装代表了根据流式细胞术预测的基因组大小 317.1 Mbp 的 95.5%。应用程序。根据 19 种蚜虫的最新基因组大小，甘氨酸包含迄今为止已知的最小的蚜虫基因组。 Ap的重复DNA含量。与Ac相比，甘氨酸基因组组装（81.6 Mbp 或 302.9 Mbp 组装的 26.94%）显示分类转座元件的数量减少。 pisum，并且可能导致估计的基因组大小较小。我们包括与宿主特异性（细胞色素 P450 和效应子）相关的基因家族的比较分析，这在Ap 中可能很重要。甘氨酸的进化。这个应用程序。 甘氨酸基因组草案序列将为研究蚜虫基因组进化、它们与寄主植物的相互作用以及新型昆虫控制方法的候选基因提供资源。