Engineering of a genetic circuit with regulatable multistability,Integrative Biology

当前位置： X-MOL 学术 › Integr. Biol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Engineering of a genetic circuit with regulatable multistability
Integrative Biology ( IF 1.5 ) Pub Date : 2018-07-24 , DOI: 10.1039/c8ib00030a
Tingting Li ₁ , Yiming Dong ₁ , Xuanqi Zhang ₁ , Xiangyu Ji ₂ , Chunxiong Luo _{1,

3} , Chunbo Lou ₂ , Haoqian M. Zhang _{1,

4} , Qi Ouyang _{1,

3}

Affiliation

Synthetic biologists are dedicated to designing genetic systems from the bottom up to understand how living systems work. To date, a variety of genetic circuits exhibiting bistability have been designed, greatly expanding our understanding of the biological multistability in natural systems. However, the study of more complex forms of biological multistability using synthetic methods is still limited. In this report, we describe the engineering of a genetic circuit with regulatable multistability. A novel genetic toggle switch exhibiting inducible bistability and a self-activation circuit were individually designed and characterized, after which they were assembled to create a circuit that presents tristability. In bacteria, this synthetic circuit enables cells to differentiate spontaneously into three different states of gene expression. Moreover, the multistability of the circuit can be modulated by external inputs. This work provides a synthetic biology framework for the study of biological multistability and may help to understand natural multistability phenomena.

中文翻译：

具有可调节的多稳定性的遗传电路工程

合成生物学家致力于从下至上设计遗传系统，以了解生物系统如何工作。迄今为止，已经设计了多种表现出双稳性的遗传回路，极大地扩展了我们对自然系统中生物多稳性的理解。但是，使用合成方法研究更复杂形式的生物多稳定性的研究仍然受到限制。在这份报告中，我们描述了具有可调节多重稳定性的遗传电路工程。分别设计和表征了新颖的具有双稳性的遗传拨动开关和自激活电路，然后组装它们以形成具有三稳性的电路。在细菌中，这种合成回路使细胞能够自发分化为三种不同的基因表达状态。而且，电路的多重稳定性可以通过外部输入进行调制。这项工作为研究生物多稳定性提供了一个综合的生物学框架，并可能有助于理解自然的多稳定性现象。

更新日期：2018-07-24

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11