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Pseudomonas sp. ST4 produces variety of active compounds to interfere fungal sexual mating and hyphal growth.
Microbial Biotechnology ( IF 4.8 ) Pub Date : 2018-06-21 , DOI: 10.1111/1751-7915.13289 Shiyin Liu 1, 2 , Fei He 1, 2 , Nuoqiao Lin 1, 2 , Yumei Chen 1, 2 , Zhibin Liang 1, 2 , Lisheng Liao 1, 2 , Mingfa Lv 1, 2 , Yufan Chen 1, 2 , Shaohua Chen 1, 2 , Jianuan Zhou 1, 2 , Lian-Hui Zhang 1, 2
Microbial Biotechnology ( IF 4.8 ) Pub Date : 2018-06-21 , DOI: 10.1111/1751-7915.13289 Shiyin Liu 1, 2 , Fei He 1, 2 , Nuoqiao Lin 1, 2 , Yumei Chen 1, 2 , Zhibin Liang 1, 2 , Lisheng Liao 1, 2 , Mingfa Lv 1, 2 , Yufan Chen 1, 2 , Shaohua Chen 1, 2 , Jianuan Zhou 1, 2 , Lian-Hui Zhang 1, 2
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Sexual mating of compatible sporida is essential for Sporisorium scitamineum to form dikaryotic mycelia and then cause infection on sugarcane. Our previous work identified a Pseudomonas sp. ST4 from a soil sample, which showed a promising biocontrol potential by inhibiting the mating of S. scitamineum sporida and hyphal growth. In this study, we set to isolate the active compounds from Pseudomonas sp. ST4 through solid fermentation. High‐performance liquid chromatography (HPLC) separation coupling with bioassay showed that Pseudomonas sp. ST4 produced a range of antimicrobial compounds. Two of the major components were purified following acetate extraction, silica gel and HPLC separation. Nuclear magnetic resonance (NMR) and liquid chromatography–mass spectrometry (LC‐MS) analysis identified these active compounds are 4‐hydroxybenzaldehyde and indole‐3‐carbaldehyde respectively. Further analysis showed that the former compound only inhibited the hyphal growth of the fungus at a concentration of 3 mM, while the latter interfered the fungal sexual mating at a concentration of 0.6 mM and affected hyphal growth at a concentration of 2 mM. Treatment of corn plants with 3 mM indole‐3‐carbaldehyde significantly inhibited corn smut infection, with a control rate up to 94%. Further analysis of the structure and activity relationship revealed that indole has a much stronger inhibitory activity against the fungal sexual mating than indole‐3‐carbaldehyde. The results from this study provide new agents for control and prevention of the sugarcane smut disease, and the active compounds could also be used to probe the molecular mechanisms of fungal sexual mating.
中文翻译：
假单胞菌 ST4产生多种活性化合物，以干扰真菌的性交和菌丝生长。
兼容的孢子虫的性交对于水孢子孢菌形成双核菌丝体然后在甘蔗上引起感染是必不可少的。我们之前的工作确定了假单胞菌属。来自土壤样品的ST4，通过抑制葡萄球菌葡萄球菌的交配和菌丝生长显示出有希望的生物防治潜力。在这项研究中，我们着手从假单胞菌sp。中分离出活性化合物。ST4通过固体发酵。高效液相色谱（HPLC）分离与生物测定联用显示假单胞菌sp。ST4生产了一系列抗菌化合物。乙酸盐萃取，硅胶和HPLC分离后，纯化两个主要成分。核磁共振（NMR）和液相色谱-质谱（LC-MS）分析确定这些活性化合物分别为4-羟基苯甲醛和吲哚-3-甲醛。进一步的分析表明，前一种化合物仅在浓度为3 mM时抑制真菌的菌丝生长，而后者在浓度为0.6 mM时会干扰真菌的性交，并在浓度为2 mM时影响菌丝的生长。用3 mM吲哚-3-甲醛处理玉米植株可显着抑制玉米黑穗病感染，控制率高达94％。对结构和活性关系的进一步分析表明，吲哚对真菌性交配的抑制活性比吲哚-3-甲醛为强。这项研究的结果为控制和预防甘蔗黑穗病提供了新的手段，并且该活性化合物还可用于探查真菌性交配的分子机制。
更新日期：2018-06-21
中文翻译：
假单胞菌 ST4产生多种活性化合物，以干扰真菌的性交和菌丝生长。
兼容的孢子虫的性交对于水孢子孢菌形成双核菌丝体然后在甘蔗上引起感染是必不可少的。我们之前的工作确定了假单胞菌属。来自土壤样品的ST4，通过抑制葡萄球菌葡萄球菌的交配和菌丝生长显示出有希望的生物防治潜力。在这项研究中，我们着手从假单胞菌sp。中分离出活性化合物。ST4通过固体发酵。高效液相色谱（HPLC）分离与生物测定联用显示假单胞菌sp。ST4生产了一系列抗菌化合物。乙酸盐萃取，硅胶和HPLC分离后，纯化两个主要成分。核磁共振（NMR）和液相色谱-质谱（LC-MS）分析确定这些活性化合物分别为4-羟基苯甲醛和吲哚-3-甲醛。进一步的分析表明，前一种化合物仅在浓度为3 mM时抑制真菌的菌丝生长，而后者在浓度为0.6 mM时会干扰真菌的性交，并在浓度为2 mM时影响菌丝的生长。用3 mM吲哚-3-甲醛处理玉米植株可显着抑制玉米黑穗病感染，控制率高达94％。对结构和活性关系的进一步分析表明，吲哚对真菌性交配的抑制活性比吲哚-3-甲醛为强。这项研究的结果为控制和预防甘蔗黑穗病提供了新的手段，并且该活性化合物还可用于探查真菌性交配的分子机制。
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