FACS index sorting allows the isolation of single cells with retrospective identification of each single cell's high‐dimensional immune phenotype. We experimentally determine the error rate of index sorting and combine the technology with T cell receptor sequencing to identify clonal T cell expansion in aplastic anemia bone marrow as an example.

中文翻译：

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FACS单细胞指数分选非常可靠，可确定克隆扩增的T细胞的免疫表型

FACS索引排序可通过回顾性鉴定每个单细胞的高维免疫表型来分离单细胞。我们以实验方式确定索引排序的错误率，并将该技术与T细胞受体测序相结合，以鉴定再生障碍性贫血骨髓中克隆性T细胞扩增。