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Imaging mRNA In Vivo, from Birth to Death
Annual Review of Biophysics ( IF 10.4 ) Pub Date : 2018-05-24 00:00:00 , DOI: 10.1146/annurev-biophys-070317-033037
Evelina Tutucci 1 , Nathan M. Livingston 2, 3 , Robert H. Singer 1, 4, 5 , Bin Wu 2, 3, 6
Affiliation  

RNA is the fundamental information transfer system in the cell. The ability to follow single messenger RNAs (mRNAs) from transcription to degradation with fluorescent probes gives quantitative information about how the information is transferred from DNA to proteins. This review focuses on the latest technological developments in the field of single-mRNA detection and their usage to study gene expression in both fixed and live cells. By describing the application of these imaging tools, we follow the journey of mRNA from transcription to decay in single cells, with single-molecule resolution. We review current theoretical models for describing transcription and translation that were generated by single-molecule and single-cell studies. These methods provide a basis to study how single-molecule interactions generate phenotypes, fundamentally changing our understating of gene expression regulation.

中文翻译:


从出生到死亡的体内mRNA成像

RNA是细胞中的基本信息传递系统。通过荧光探针跟踪单个信使RNA(mRNA)从转录到降解的能力提供了有关信息如何从DNA转移到蛋白质的定量信息。这篇综述着重于单mRNA检测领域的最新技术发展及其在固定细胞和活细胞中研究基因表达的用途。通过描述这些成像工具的应用,我们追踪了单分子分辨率下单细胞中mRNA从转录到衰变的过程。我们回顾了用于描述单分子和单细胞研究产生的转录和翻译的当前理论模型。这些方法为研究单分子相互作用如何产生表型提供了基础,

更新日期:2018-05-24
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