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Artificial MicroRNAs Promote High-Level Production of Biomolecules Through Metabolic Engineering of Phenylpropanoid Pathway
Critical Reviews in Plant Sciences ( IF 6.0 ) Pub Date : 2018-03-16 , DOI: 10.1080/07352689.2018.1444361
Sweda Sreekumar 1 , E. V. Soniya 1
Affiliation  

Plant microRNAs (miRNAs) are tiny RNAs that are crucial for the biosynthesis of secondary metabolites such as phenylpropanoids because they regulate the activity of key transcription factors and enzymes. Here, we have discussed the significance of miRNAs that target the mRNAs encoding for enzymes and transcription factors in the phenylpropanoid pathway. Genes encoding the miRNAs involved in the synthesis of phenylpropanoids could be considered as potential candidates for metabolic engineering. We have analyzed the construction and potential value of artificial miRNAs (amiRNAs) that could be used for scrutinizing the functional importance of miRNAs in phenylpropanoid biosynthesis and manipulating the pathway for enhancing the production of secondary metabolites.

中文翻译:

人工MicroRNA通过苯丙烷途径的代谢工程促进生物分子的高水平生产。

植物microRNA(miRNA)是微小的RNA,对于次级代谢产物(如苯丙烷)的生物合成至关重要,因为它们调节关键转录因子和酶的活性。在这里,我们讨论了靶向于编码苯丙氨酸途径中的酶和转录因子的mRNA的miRNA的重要性。编码参与苯丙氨酸合成的miRNA的基因可以被认为是代谢工程的潜在候选者。我们已经分析了人工miRNA(amiRNA)的构建和潜在价值,这些miRNA可用于研究miRNA在苯丙烷类生物合成中的功能重要性,并操纵增强次生代谢产物生产的途径。
更新日期:2018-07-14
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