Immediate hemorrhage control and anti-infection play important roles in the wound management. Besides, a moist environment is also beneficial for wound healing. Hydrogels are promising materials in urgent hemostasis and drug release. However, hydrogels have the disadvantage of rapid release profiles, leading to the exposure to high drug concentrations. In this study, we constructed hybrid hydrogels with rapid hemostasis and sustainable antibacterial property combining aminoethyl methacrylate hyaluronic acid (HA-AEMA) and methacrylated methoxy polyethylene glycol (mPEG-MA) hybrid hydrogels and chlorhexidine diacetate (CHX)-loaded nanogels. The CHX-loaded nanogels (CLNs) were prepared by the enzyme degradation of CHX-loaded lysine-based hydrogels. The HA-AEMA and mPEG-MA hybrid hydrogel loaded with CLNs (labeled as [email protected]) displayed a three-dimensional microporous structure and exhibited excellent swelling, mechanical property, and low cytotoxicity. The [email protected] hydrogel showed a prolonged release period of CHX over 240 h and the antibacterial property over 10 days. The hemostasis and wound-healing properties were evaluated in vivo using a mouse model. The results showed that hydrogel had the rapid hemostasis capacity and accelerated wound healing. In summary, CLN-loaded hydrogels may be excellent candidates as hemostasis and anti-infection materials for the wound dressing application.

中文翻译：

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透明质酸和聚乙二醇杂化水凝胶包封具有止血和可持续抗菌特性的纳米凝胶，用于伤口愈合

立即的出血控制和抗感染在伤口处理中起着重要的作用。此外，潮湿的环境也有利于伤口愈合。水凝胶是紧急止血和药物释放的有前途的材料。但是，水凝胶的缺点是释放速度快，导致暴露于高药物浓度。在这项研究中，我们构建了具有快速止血和可持续抗菌性能的杂化水凝胶，结合了甲基丙烯酸氨基乙酯透明质酸（HA-AEMA）和甲基丙烯酸甲氧基聚乙二醇（mPEG-MA）杂化水凝胶和载有双氯乙酸洗必泰（CHX）的纳米凝胶。CHX负载的纳米凝胶（CLNs）是通过对CHX负载的赖氨酸基水凝胶进行酶降解来制备的。装有CLN（标记为[电子邮件保护]）的HA-AEMA和mPEG-MA杂化水凝胶显示三维微孔结构，并具有出色的溶胀，机械性能和低细胞毒性。受[电子邮件保护]的水凝胶在240小时内显示出CHX延长的释放时间，并在10天内显示出抗菌性能。使用小鼠模型在体内评估止血和伤口愈合特性。结果表明，水凝胶具有快速的止血能力和加速的伤口愈合。总之，负载CLN的水凝胶可能是伤口敷料应用中的止血和抗感染材料的极佳候选者。受[电子邮件保护]的水凝胶在240小时内显示出CHX延长的释放时间，并在10天内显示出抗菌性能。使用小鼠模型在体内评估止血和伤口愈合特性。结果表明，水凝胶具有快速的止血能力和加速的伤口愈合。总之，负载CLN的水凝胶可能是伤口敷料应用中的止血和抗感染材料的极佳候选者。受[电子邮件保护]的水凝胶在240小时内显示出CHX延长的释放时间，并在10天内显示出抗菌性能。使用小鼠模型在体内评估止血和伤口愈合特性。结果表明，水凝胶具有快速的止血能力和加速的伤口愈合。总之，负载CLN的水凝胶可能是伤口敷料应用中的止血和抗感染材料的极佳候选者。