Slx5p-Slx8p Promotes Accurate Chromosome Segregation by Mediating the Degradation of Synaptonemal Complex Components during Meiosis.,Advanced Science

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Slx5p-Slx8p Promotes Accurate Chromosome Segregation by Mediating the Degradation of Synaptonemal Complex Components during Meiosis.
Advanced Science ( IF 14.3 ) Pub Date : 2020-01-01 , DOI: 10.1002/advs.201900739
Chao Liu ₁ , Haichao Zhao _{1,

2} , Sai Xiao _{1,

2} , Tingting Han ₃ , Yinghong Chen _{1,

2} , Tong Wang ₁ , Yanjie Ma _{1,

2} , Hui Gao ₁ , Zhiping Xie ₄ , Li-Lin Du ₅ , Jian Li ₃ , Guoping Li _{1,

3} , Wei Li _{1,

2}

Affiliation

Meiosis increases genetic diversity, yet the genome complement needs to be stable to ensure offspring viability. Both small ubiquitin-like modifier (SUMO) and ubiquitin have been reported to participate in meiotic regulation, yet functions of the SUMO-ubiquitination crosstalk in meiosis remain unclear. Here, it is reported that a SUMO-targeted ubiquitin ligase, Slx8p, promotes accurate chromosome segregation during meiosis, since the deletion of SLX8 leads to increased aneuploidy due to a defect in synaptonemal complex (SC) component degradation. Both the RING domain and SUMO interacting motifs of Slx8p are essential for meiotic progression and maintaining spore viability, and the expression of tetraubiquitin fused with SUMO partially rescues meiotic defects in the SLX8-deletion strain. Furthermore, Slx5p-Slx8p can directly add ubiquitin to SUMOylated Zip1p and Ecm11p, and forced degradation of Ecm11p partially rescues the sporulation defects of the SLX8 deletion strain. These findings provide a mechanism for SC disassembly and reveal that the crosstalk between SUMOylation and ubiquitination facilitates accurate chromosome segregation by promoting SC component degradation during meiosis.

中文翻译：

Slx5p-Slx8p 通过介导减数分裂期间联会复合体成分的降解来促进准确的染色体分离。

减数分裂增加了遗传多样性，但基因组补充需要稳定以确保后代的生存能力。据报道，小泛素样修饰剂（SUMO）和泛素都参与减数分裂调节，但 SUMO 泛素化串扰在减数分裂中的功能仍不清楚。据报道，SUMO 靶向泛素连接酶 Slx8p 可促进减数分裂过程中染色体的准确分离，因为删除 SLX8 会因联会复合体 (SC) 成分降解缺陷而导致非整倍性增加。 Slx8p 的 RING 结构域和 SUMO 相互作用基序对于减数分裂进程和维持孢子活力至关重要，与 SUMO 融合的四泛素的表达部分挽救了 SLX8 缺失菌株的减数分裂缺陷。此外，Slx5p-Slx8p可以直接向SUMO化的Zip1p和Ecm11p添加泛素，并且Ecm11p的强制降解部分挽救了SLX8缺失菌株的孢子形成缺陷。这些发现提供了 SC 分解的机制，并揭示了 SUMO 化和泛素化之间的串扰通过促进减数分裂过程中 SC 成分的降解来促进准确的染色体分离。

更新日期：2020-01-02

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