In this study, an injectable and self-healing hydrogel based on the boronic ester dynamic covalent bond between phenylboronic acid modified hyaluronic acid (HA-PBA) and the commercially available poly (vinyl alcohol) (PVA) is prepared and should have multi-functions for biomedical applications. The hydrogels were rapidly formed under mild conditions, and the rheological properties and in vitro degradation were systematically characterized. The HA-based hydrogels possessed good injectability and self-healing properties because of the dynamic bond. Moreover, due to the sensitivity of boronic ester to the biologically relevant concentration of hydrogen peroxide (H2O2), a major reactive oxygen species (ROS), the injectable hydrogel could be used as a H2O2/ROS responsive drug delivery system. The hydrogels supported good viability of encapsulated neural progenitor cells (NPC) and protected NPC from ROS induced damage in vitro when H2O2 was present in the media. The dynamic hydrogels were further applied as bio-inks for 3D printing/bioprinting. Overall, this facilely prepared dynamic hydrogel based on HA-PBA and PVA may have many potential biomedical applications, including drug delivery, 3D culture of cells, and 3D bioprinting.

中文翻译：

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通用动态透明质酸基水凝胶的制备。

在这项研究中，基于苯基硼酸改性的透明质酸（HA-PBA）与市售的聚乙烯醇（PVA）之间的硼酸酯动态共价键，制备了一种可注射且能自我修复的水凝胶，并且应具有多种功能用于生物医学应用。水凝胶在温和条件下迅速形成，并系统地表征了流变性质和体外降解。由于动态键合，基于HA的水凝胶具有良好的可注射性和自愈特性。此外，由于硼酸酯对生物学相关浓度的过氧化氢（H2O2），主要的活性氧（ROS）的敏感性，因此可注射水凝胶可用作H2O2 / ROS响应型药物递送系统。当介质中存在H2O2时，水凝胶支持封装的神经祖细胞（NPC）的良好生存力，并保护NPC免受ROS诱导的体外损伤。动态水凝胶进一步用作3D打印/生物打印的生物油墨。总体而言，这种基于HA-PBA和PVA的简便制备的动态水凝胶可能具有许多潜在的生物医学应用，包括药物递送，细胞的3D培养和3D生物打印。