The faithful inheritance of chromosomes is essential for the propagation of organisms. In eukaryotes, central to this process is the mitotic spindle. Recently, we have identified TRIM8 as a gene aberrantly expressed in gliomas whose expression reduces the clonogenic potential in the patients' glioma cells. TRIM8 encodes an E3 ubiquitin ligase involved in various pathological processes, including hypertrophy, antiviral defense, encephalopathy, and cancer development. To gain insights into the TRIM8 functions, we characterized the TRIM8 interactome in primary mouse embryonic neural stem cells using proteomics. We found that TRIM8 interacts with KIFC1, and KIF11/Eg5, two master regulators of mitotic spindle assembly and cytoskeleton reorganization. By exploring the TRIM8 role in the mitotic spindle machinery, we showed that TRIM8 localizes at the mitotic spindle during mitosis and plays a role in centrosome separation at the beginning of mitosis with a subsequent delay of the mitotic progression and impact on chromosomal stability.

中文翻译：

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TRIM8与KIF11和KIFC1相互作用，并控制双极纺锤体的形成和染色体稳定性。

染色体的忠实遗传对生物繁殖至关重要。在真核生物中，此过程的中心是有丝分裂纺锤体。最近，我们已经确定TRIM8是在神经胶质瘤中异常表达的基因，其表达降低了患者神经胶质瘤细胞的克隆形成潜能。TRIM8编码一个E3泛素连接酶，参与各种病理过程，包括肥大，抗病毒防御，脑病和癌症发展。为了深入了解TRIM8功能，我们使用蛋白质组学对了TRIM8相互作用基因组在原代小鼠胚胎神经干细胞中进行了表征。我们发现TRIM8与有丝分裂纺锤体组装和细胞骨架重组的两个主要调控因子KIFC1，KIF11 / Eg5相互作用。通过探索TRIM8在有丝分裂纺锤体机械中的作用，