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Abnormal expression of rno_circRNA_014900 and rno_circRNA_005442 induced by ketamine in the rat hippocampus.
BMC Psychiatry ( IF 4.4 ) Pub Date : 2020-01-02 , DOI: 10.1186/s12888-019-2374-2 Jing Mao 1 , Tianmei Li 1 , Di Fan 1 , Hongli Zhou 1 , Jianguo Feng 2 , Li Liu 3 , Chunxiang Zhang 4 , Xiaobin Wang 3
BMC Psychiatry ( IF 4.4 ) Pub Date : 2020-01-02 , DOI: 10.1186/s12888-019-2374-2 Jing Mao 1 , Tianmei Li 1 , Di Fan 1 , Hongli Zhou 1 , Jianguo Feng 2 , Li Liu 3 , Chunxiang Zhang 4 , Xiaobin Wang 3
Affiliation
BACKGROUND
Recent studies have shown that circular RNA (circRNA) is rich in microRNA (miRNA) binding sites. We have previously demonstrated that the antidepressant effect of ketamine is related to the abnormal expression of various miRNAs in the brain. This study determined the expression profile of circRNAs in the hippocampus of rats treated with ketamine.
METHODS
The aberrantly expressed circRNAs in rat hippocampus after ketamine injection were analyzed by microarray chip, and we further validated these circRNAs by quantitative reverse-transcription PCR (qRT-PCR). The target genes of the different circRNAs were predicted using bioinformatic analyses, and the functions and signal pathways of these target genes were investigated by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses.
RESULTS
Microarray analysis showed that five circRNAs were aberrantly expressed in rat hippocampus after ketamine injection (fold change > 2.0, p < 0.05). The results from the qRT-PCR showed that one of the circRNAs was significantly increased (rno_circRNA_014900; fold change = 2.37; p = 0.03), while one was significantly reduced (rno_circRNA_005442; fold change = 0.37; p = 0.01). We discovered a significant enrichment in several GO terms and pathways associated with depression.
CONCLUSION
Our findings showed the abnormal expression of ketamine-induced hippocampal circRNAs in rats.
中文翻译：
氯胺酮诱导的大鼠海马中rno_circRNA_014900和rno_circRNA_005442的异常表达。
背景技术最近的研究表明，环状RNA（circRNA）富含microRNA（miRNA）结合位点。我们以前已经证明了氯胺酮的抗抑郁作用与大脑中各种miRNA的异常表达有关。这项研究确定了氯胺酮治疗的大鼠海马中circRNA的表达特征。方法用微阵列芯片分析氯胺酮注射后大鼠海马中异常表达的circRNA，并通过定量逆转录PCR（qRT-PCR）进一步验证这些circRNA。使用生物信息学分析预测了不同circRNA的靶基因，并通过基因本体论（GO）和《京都基因与基因组百科全书》（KEGG）途径分析了这些靶基因的功能和信号途径。结果微阵列分析显示，氯胺酮注射后大鼠海马中有五个circRNA异常表达（倍数变化> 2.0，p <0.05）。来自qRT-PCR的结果表明，其中一种circRNA显着增加（rno_circRNA_014900;倍数变化= 2.37; p = 0.03），而其中一种明显降低（circ_circRNA_005442;倍数变化= 0.37; p = 0.01）。我们发现与抑郁症相关的几个GO术语和途径显着丰富。结论我们的发现表明氯胺酮诱导的大鼠海马circRNA的异常表达。p = 0.03），而其中一个显着降低（rno_circRNA_005442；倍数变化= 0.37； p = 0.01）。我们发现与抑郁症相关的几个GO术语和途径显着丰富。结论我们的发现表明氯胺酮诱导的大鼠海马circRNA的异常表达。p = 0.03），而其中一个显着降低（rno_circRNA_005442；倍数变化= 0.37； p = 0.01）。我们发现与抑郁症相关的几个GO术语和途径显着丰富。结论我们的发现表明氯胺酮诱导的大鼠海马circRNA的异常表达。
更新日期：2020-01-02
中文翻译：
氯胺酮诱导的大鼠海马中rno_circRNA_014900和rno_circRNA_005442的异常表达。
背景技术最近的研究表明，环状RNA（circRNA）富含microRNA（miRNA）结合位点。我们以前已经证明了氯胺酮的抗抑郁作用与大脑中各种miRNA的异常表达有关。这项研究确定了氯胺酮治疗的大鼠海马中circRNA的表达特征。方法用微阵列芯片分析氯胺酮注射后大鼠海马中异常表达的circRNA，并通过定量逆转录PCR（qRT-PCR）进一步验证这些circRNA。使用生物信息学分析预测了不同circRNA的靶基因，并通过基因本体论（GO）和《京都基因与基因组百科全书》（KEGG）途径分析了这些靶基因的功能和信号途径。结果微阵列分析显示，氯胺酮注射后大鼠海马中有五个circRNA异常表达（倍数变化> 2.0，p <0.05）。来自qRT-PCR的结果表明，其中一种circRNA显着增加（rno_circRNA_014900;倍数变化= 2.37; p = 0.03），而其中一种明显降低（circ_circRNA_005442;倍数变化= 0.37; p = 0.01）。我们发现与抑郁症相关的几个GO术语和途径显着丰富。结论我们的发现表明氯胺酮诱导的大鼠海马circRNA的异常表达。p = 0.03），而其中一个显着降低（rno_circRNA_005442；倍数变化= 0.37； p = 0.01）。我们发现与抑郁症相关的几个GO术语和途径显着丰富。结论我们的发现表明氯胺酮诱导的大鼠海马circRNA的异常表达。p = 0.03），而其中一个显着降低（rno_circRNA_005442；倍数变化= 0.37； p = 0.01）。我们发现与抑郁症相关的几个GO术语和途径显着丰富。结论我们的发现表明氯胺酮诱导的大鼠海马circRNA的异常表达。
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