C. maxima (var. Delica), a variety of pumpkin, is well known for its high concentration on carotenoids, possessing dietary benefits and antioxidant properties. Aflatoxins and enniatins are common mycotoxins present in food and feed with an extended toxicity profile in humans and animals. Both types of substances reach a wide range of tissues and organs and have the capability to penetrate the blood brain barrier. Since carotenoids and mycotoxins have been reported to modify diverse mitochondrial processes individually, transcriptional in vitro studies on human epithelial cells ECV 304 were conducted to analyze the relative expression of 13 mitochondria related genes. ECV 304 cells were differentiated for 9 days and treated for 2 h with: a) pumpkin (500 nM); b) aflatoxins (100 nM); c) enniatins (100 nM); d) aflatoxins (100 nM) and pumpkin (500 nM); e) enniatins (100 nM) and pumpkin (500 nM). Even at low concentrations, dietary carotenoids activity on mitochondrial genes expression reported a beneficial effect and, for most of the genes studied across the Electron Transport Chain (ETC), developed a protective effect when mixed with aflatoxins (AFs) or enniatins (ENs).

中文翻译：

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线粒体转录研究在血脑屏障模型中体外黄曲霉毒素，烯醇核苷和类胡萝卜素作用的研究。

C. maxima（var。Delica）是一种南瓜，以其对类胡萝卜素的高浓度而闻名，具有饮食益处和抗氧化特性。黄曲霉毒素和烯醇核苷是食物和饲料中常见的霉菌毒素，在人和动物中的毒性范围扩大。两种类型的物质都能到达广泛的组织和器官，并具有穿透血脑屏障的能力。由于据报道类胡萝卜素和霉菌毒素分别改变了各种线粒体过程，因此对人上皮细胞ECV 304进行了体外转录研究，以分析13种线粒体相关基因的相对表达。将ECV 304细胞分化9天，并用以下方法处理2小时：a）南瓜（500 nM）；b）黄曲霉毒素（100 nM）; c）肾上腺素（100 nM）; d）黄曲霉毒素（100 nM）和南瓜（500 nM）；e）恩尼丁（100 nM）和南瓜（500 nM）。即使在低浓度下，饮食类胡萝卜素对线粒体基因表达的活性也显示出有益的作用，并且对于跨电子传输链（ETC）研究的大多数基因，当与黄曲霉毒素（AFs）或enenatins（ENs）混合时，都具有保护作用。