The 40 amino acid residues peptide MciZ (Mother Cell Inhibitor of FtsZ) represents a promising antibacterial agent as it is an effective inhibitor of bacterial cell division, Z-ring formation and localization. However, its efficacy is limited to Gram-positive bacteria as MciZ is unable to penetrate into Gram-negative organisms. In this study, we report the synthesis of plasmonic silver chloride nanoparticles stabilized by MciZ (NP/MciZ). NP/MciZ were synthesized using a fast and green route under blue light irradiation. Electron microscopy showed that NP/MciZ were enveloped by a peptide layer responsible for colloidal stability. X-ray diffraction analysis showed that silver chloride nanoparticles were crystalline in nature with small proportion of metallic silver. NP/MciZ showed a dose-dependent cytotoxicity against mammalian VERO cells. However, NP/MciZ exhibited remarkable antibacterial activity against Gram-positive bacterium B. megaterium comparable to MciZ. Furthermore, NP/MciZ showed similar antimicrobial activity against the Gram-negative bacterium E. coli and the yeast C. albicans. The photochemically-generated NP/MciZ presented here is a new organic–inorganic hybrid nanomaterial and has potential for biomedical or other applications.

中文翻译：

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光化学生成的氯化银纳米粒子的细胞分裂肽抑制剂稳定及其抗菌特性

的40个氨基酸残基肽MCIZ（中号其他ç ELL我nhibitor FTS的ž）代表一种有前途的抗菌剂，因为它是细菌细胞分裂，Z环形成和定位的有效抑制剂。但是，由于MciZ无法渗透到革兰氏阴性菌中，因此其功效仅限于革兰氏阳性菌。在这项研究中，我们报告了由MciZ（NP / MciZ）稳定的等离激元氯化银纳米粒子的合成。NP / MciZ是在蓝光照射下使用快速且绿色的路线合成的。电子显微镜显示NP / MciZ被负责胶体稳定性的肽层所包裹。X射线衍射分析表明，氯化银纳米颗粒本质上是结晶的，而金属银的比例很小。NP / MciZ对哺乳动物VERO细胞显示出剂量依赖性的细胞毒性。然而，与MciZ相当的巨大芽孢杆菌。此外，NP / MciZ对革兰氏阴性菌大肠杆菌和白色念珠菌也有相似的抗菌活性。本文介绍的光化学生成的NP / MciZ是一种新型的有机-无机杂化纳米材料，具有生物医学或其他应用的潜力。