Ankylosing spondylitis (AS) is a progressive systemic disease characterized by chronic inflammation response of the sacroiliac joint and spine. Long non-coding RNAs (lncRNAs) are widely involved in the regulation of various diseases. However, the role of lncRNA maternally expressed gene 3 (MEG3) in the inflammatory response of AS has not been studied. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of inflammatory cytokines interleukin-1β (IL-1β) and interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in tissues and cells. The expression levels of MEG3, microRNA-146a (miR-146a), and inflammatory cytokines were measured by quantitative real-time PCR (qRT-PCR). Correlation between MEG3 or miR-146a and inflammatory cytokines was analyzed by Pearson analysis. Dual-luciferase reporter and RNA immunoprecipitation (RIP) assays were used to clarify the interaction between MEG3 and miR-146a. MEG3 was downregulated in AS patients, negatively correlated with the levels of IL-1β, IL-6, and TNF-α, and blocked the inflammatory response of AS. MiR-146a was upregulated in AS patients and could interact with MEG3. The expression of miR-146a was positively correlated with IL-1β, IL-6, and TNF-α levels. Overexpression of miR-146a reversed the inhibitory effect of abnormal MEG3 expression on inflammatory cytokines. LncRNA MEG3 plays an anti-inflammatory role in AS partially through targeting miR-146a, which provides a potential new means for the treatment of AS patients.

中文翻译：

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LncRNA MEG3 通过靶向 miR-146a 抑制强直性脊柱炎的炎症反应。

强直性脊柱炎（AS）是一种进行性全身性疾病，其特征是骶髂关节和脊柱的慢性炎症反应。长链非编码 RNA (lncRNA) 广泛参与各种疾病的调控。然而，尚未研究 lncRNA 母体表达基因 3（MEG3）在 AS 炎症反应中的作用。酶联免疫吸附试验（ELISA）用于检测组织和细胞中炎性细胞因子白细胞介素1β（IL-1β）和白细胞介素6（IL-6）和肿瘤坏死因子-α（TNF-α）的水平。通过定量实时 PCR (qRT-PCR) 测量 MEG3、microRNA-146a (miR-146a) 和炎性细胞因子的表达水平。通过 Pearson 分析分析 MEG3 或 miR-146a 与炎性细胞因子之间的相关性。双荧光素酶报告基因和 RNA 免疫沉淀 (RIP) 分析用于阐明 MEG3 和 miR-146a 之间的相互作用。MEG3 在 AS 患者中下调，与 IL-1β、IL-6 和 TNF-α 水平呈负相关，并阻断 AS 的炎症反应。MiR-146a 在 AS 患者中上调并且可以与 MEG3 相互作用。miR-146a的表达与IL-1β、IL-6和TNF-α水平呈正相关。miR-146a 的过表达逆转了 MEG3 异常表达对炎性细胞因子的抑制作用。LncRNA MEG3部分通过靶向miR-146a在AS中发挥抗炎作用，为AS患者的治疗提供了一种潜在的新手段。与 IL-1β、IL-6 和 TNF-α 水平呈负相关，并阻断 AS 的炎症反应。MiR-146a 在 AS 患者中上调并且可以与 MEG3 相互作用。miR-146a的表达与IL-1β、IL-6和TNF-α水平呈正相关。miR-146a 的过表达逆转了 MEG3 异常表达对炎性细胞因子的抑制作用。LncRNA MEG3部分通过靶向miR-146a在AS中发挥抗炎作用，为AS患者的治疗提供了一种潜在的新手段。与 IL-1β、IL-6 和 TNF-α 水平呈负相关，并阻断 AS 的炎症反应。MiR-146a 在 AS 患者中上调并且可以与 MEG3 相互作用。miR-146a的表达与IL-1β、IL-6和TNF-α水平呈正相关。miR-146a 的过表达逆转了 MEG3 异常表达对炎性细胞因子的抑制作用。LncRNA MEG3部分通过靶向miR-146a在AS中发挥抗炎作用，为AS患者的治疗提供了一种潜在的新手段。miR-146a 的过表达逆转了 MEG3 异常表达对炎性细胞因子的抑制作用。LncRNA MEG3部分通过靶向miR-146a在AS中发挥抗炎作用，为AS患者的治疗提供了一种潜在的新手段。miR-146a 的过表达逆转了 MEG3 异常表达对炎性细胞因子的抑制作用。LncRNA MEG3部分通过靶向miR-146a在AS中发挥抗炎作用，为AS患者的治疗提供了一种潜在的新手段。