Baculoviruses have been developed as long-term and environmentally friendly biopesticides. However, solar ultraviolet radiation can reduce the activity of baculovirus. Radiation sensitive 23 (Rad23) can recognize DNA damage and is involved with nucleotide excision repair (NER). In the current study, BmRad23 was accumulated mainly within the nucleus. Host cell reactivation (HCR) assays have shown that BmRad23 significantly facilitated the expression of UV-damaged mCherry reporter gene. Reverse transcription quantitative PCR (RT-qPCR) result showed that the mRNA expression level of BmRad23 was increased in (Bombyx mori nuclear polyhedrovirus, BmNPV) BmNPV-infected BmN cells. However, the expression of BmRad23 was increased significantly when BmNPV budded viruses (BVs) or BmN cells were irradiated with UV light. Overexpression of BmRad23 promoted the mRNA levels of two UV-induced DNA damage repair genes which were from Bombyx mori and BmNPV, respectively. Meanwhile, the overexpression of BmRad23 in BmN cells was conducive to the proliferation of BmNPV and UV-damaged BmNPV. The recombinant BmNPV BVs expressing BmRad23 showed stronger resistance to UV radiation than the control virus. In conclusion, the results revealed that BmRad23 contributed to the proliferation of BmNPV and the repair of UV-damaged BmNPV, which would provide a reference for the development of efficient baculovirus pesticides against UV radiation.

中文翻译：

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Bombyx mori Rad23 (BmRad23) 有助于修复受紫外线损伤的 BmNPV

杆状病毒已被开发为长期和环境友好的生物农药。然而，太阳紫外线辐射可以降低杆状病毒的活性。辐射敏感 23 (Rad23) 可以识别 DNA 损伤并参与核苷酸切除修复 (NER)。在目前的研究中，BmRad23 主要在细胞核内积累。宿主细胞再激活 (HCR) 分析表明 BmRad23 显着促进了紫外线损伤的 mCherry 报告基因的表达。逆转录定量 PCR (RT-qPCR) 结果表明，BmRad23 的 mRNA 表达水平在（家蚕核多角体病毒，BmNPV）BmNPV 感染的 BmN 细胞中增加。然而，当用紫外线照射 BmNPV 出芽病毒 (BV) 或 BmN 细胞时，BmRad23 的表达显着增加。BmRad23 的过表达促进了两个分别来自家蚕和 BmNPV 的紫外线诱导的 DNA 损伤修复基因的 mRNA 水平。同时，BmRad23在BmN细胞中的过表达有利于BmNPV和UV损伤的BmNPV的增殖。表达 BmRad23 的重组 BmNPV BV 表现出比对照病毒更强的抗紫外线辐射能力。综上所述，结果表明BmRad23促进了BmNPV的增殖和紫外线损伤BmNPV的修复，为开发抗紫外线辐射的高效杆状病毒杀虫剂提供了参考。表达 BmRad23 的重组 BmNPV BV 表现出比对照病毒更强的抗紫外线辐射能力。综上所述，结果表明BmRad23有助于BmNPV的增殖和紫外线损伤BmNPV的修复，为开发高效抗紫外线辐射的杆状病毒杀虫剂提供参考。表达 BmRad23 的重组 BmNPV BV 表现出比对照病毒更强的抗紫外线辐射能力。综上所述，结果表明BmRad23有助于BmNPV的增殖和紫外线损伤BmNPV的修复，为开发高效抗紫外线辐射的杆状病毒杀虫剂提供参考。