Abstract The expression of anti-freeze proteins (AFPs) on the Escherichia coli using the cell surface display (CSD) strategy was investigated to determine the feasibility of the method as a potential application of kinetic hydrate inhibitors. Monomers to hexamers of type II and III AFPs were displayed on the surfaces of bacterial cells. The kinetic inhibition performance of the AFPs on the synthetic natural gas hydrate formation was tested. The displayed AFPs exhibited higher KHI effectiveness with the increase in the number of peptides attached to the cells. In addition, best KHI performance of the AFPs could be achieved with higher dosages of the cultures when the concentration of the inducer is optimized, regardless of AFP type. Only a small portion of AFPs in the cell cultures exhibited considerable KHI performance, which implies that the CSD could be an effective strategy, reducing the cost for the production and purification of the AFPs.

中文翻译：

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来自 Clupea harangues 和 Anarhichas minor 的抗冻蛋白通过细胞表面显示对气体水合物抑制的功效

摘要 使用细胞表面展示 (CSD) 策略研究了抗冻蛋白 (AFP) 在大肠杆菌上的表达，以确定该方法作为动力学水合物抑制剂的潜在应用的可行性。II 型和 III 型 AFP 的单体到六聚体显示在细菌细胞表面。测试了AFPs对合成天然气水合物形成的动力学抑制性能。随着附着在细胞上的肽数量的增加，展示的 AFP 表现出更高的 KHI 有效性。此外，当优化诱导剂的浓度时，无论 AFP 类型如何，都可以通过更高剂量的培养物实现 AFP 的最佳 KHI 性能。细胞培养物中只有一小部分 AFP 表现出可观的 KHI 性能，