Abstract An analytical method was established to simultaneously determine prochloraz and three metabolites N-propyl-N-[2-(2,4,6-trichlorophenoxy)ethyl] urea(BTS44595), N-formyl-N-propyl-N-[2-(2,4,6-trichlorophenoxy)ethyl] urea(BTS44596), and 2,4,6-trichlorophenol(BTS45186) in fruits and vegetables. The analysis was performed by liquid chromatography – tandem mass spectrometry (LC-MS/MS) in the positive and negative ion-switching multiple reaction modes (MRM). The analytes were extracted using acetonitrile. The extract was further purified by dispersive solid phase extraction. The linearity of the matrix-matched calibration curves was verified with correlation coefficients (r) greater than 0.9975. Recoveries in fruits and vegetables were conducted at four appropriate concentrations. The average recoveries of the method ranged from 80% to 105%, and the relative standard deviations were less than 15%. The limits of detection and quantification of the analytes in fruits and vegetables were from 0.1 to 1 µg/kg and 0.5 to 5 µg/kg, respectively. The method was validated and demonstrated to be suitable for the determination of prochloraz and three metabolites in fruits and vegetables.

中文翻译：

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液相色谱-串联质谱法 (LC-MS/MS) 同时测定水果和蔬菜中的丙氯灵和三种代谢物

摘要 建立了同时测定丙氯灵和三种代谢物N-丙基-N-[2-(2,4,6-三氯苯氧基)乙基]脲(BTS44595)、N-甲酰基-N-丙基-N-[2 -(2,4,6-三氯苯氧基)乙基]脲(BTS44596)和水果和蔬菜中的2,4,6-三氯苯酚(BTS45186)。分析是通过液相色谱-串联质谱 (LC-MS/MS) 在正负离子切换多反应模式 (MRM) 下进行的。使用乙腈提取分析物。通过分散固相萃取进一步纯化提取物。基质匹配校准曲线的线性经过验证，相关系数 (r) 大于 0.9975。水果和蔬菜中的回收率在四种合适的浓度下进行。该方法的平均回收率为 80% 至 105%，相对标准偏差小于15%。水果和蔬菜中分析物的检测限和定量限分别为 0.1 至 1 µg/kg 和 0.5 至 5 µg/kg。该方法经过验证并证明适用于测定水果和蔬菜中的丙氯灵和三种代谢物。