Biomaterial integration into bone requires optimal surface conditions to promote osteoprogenitor behavior, which is affected by integrin‐binding via arginine‐glycine‐aspartate (RGD). RGD‐functionalized supported lipid bilayers (SLBs) might be interesting as biomaterial coating in bone regeneration, because they allow integration of proteins, for example, growth factors, cytokines, and/or antibacterial agents. Since it is unknown whether and how they affect osteoprogenitor adhesion and differentiation, the aim was to investigate adhesion, focal adhesion formation, morphology, proliferation, and osteogenic potential of pre‐osteoblasts cultured on RGD‐functionalized SLBs compared to unfunctionalized SLBs and poly‐l‐lysine (PLL). After 17 hr, pre‐osteoblast density on SLBs without or with RGD was similar, but lower than on PLL. Cell surface area, elongation, and number and size of phospho‐paxillin clusters were also similar. Cells on SLBs without or with RGD were smaller, more elongated, and had less and smaller phospho‐paxillin clusters than on PLL. OPN expression was increased on SLBs with RGD compared to PLL. Moreover, after 1 week, COL1a1 expression was increased on SLBs without or with RGD. In conclusion, pre‐osteoblast adhesion and enhanced differentiation were realized for the first time on RGD‐functionalized SLBs, pointing to a new horizon in the management of bone regeneration using biomaterials. Together with SLBs nonfouling nature and the possibility of adjusting SLB fluidity and peptide content make SLBs highly promising as substrate to develop innovative biomimetic coatings for biomaterials in bone regeneration.

中文翻译：

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RGD 功能化支持的脂质双层调节前成骨细胞粘附并促进成骨分化。

生物材料整合到骨骼中需要最佳的表面条件来促进骨祖细胞的行为，而骨祖细胞的行为受精氨酸-甘氨酸-天冬氨酸 (RGD) 整合素结合的影响。RGD 功能化的支持脂质双层 (SLB) 作为骨再生中的生物材料涂层可能很有趣，因为它们允许整合蛋白质，例如生长因子、细胞因子和/或抗菌剂。由于尚不清楚它们是否以及如何影响骨祖细胞粘附和分化，目的是研究在 RGD 功能化 SLB 上培养的前成骨细胞与未功能化 SLB 和多聚L 的粘附、粘着斑形成、形态学、增殖和成骨潜力。赖氨酸 (PLL)。17 小时后，无或有 RGD 的 SLB 上的前成骨细胞密度相似，但低于 PLL。细胞表面积、伸长率以及磷酸桩蛋白簇的数量和大小也相似。与 PLL 相比，没有或有 RGD 的 SLB 上的细胞更小、更长，并且磷酸桩蛋白簇越来越小。与 PLL 相比，具有 RGD 的 SLB 上的OPN表达增加。此外，1周后，COL1a1在没有或有 RGD 的情况下，SLB 上的表达增加。总之，首次在 RGD 功能化的 SLB 上实现了前成骨细胞粘附和增强的分化，为使用生物材料管理骨再生开辟了新的视野。加上 SLB 的无污染性质以及调节 SLB 流动性和肽含量的可能性，使 SLB 非常有希望作为基材开发用于骨再生生物材料的创新仿生涂层。