In the present work, a novel strategy was explored to fabricate nanofiber scaffolds consisting of cellulose assimilated with titanium dioxide (TiO₂) and silver (Ag) nanoparticles (NPs). The concentration of the TiO₂ NPs in the composite was adjusted to 1.0, 1.5, and 2.0 wt % with respect to polymer concentration used for the electrospinning of colloidal solutions. The fabricated composite scaffolds were dispensed to alkaline deacetylation using 0.05 M NaOH to remove the acetyl groups in order to generate pure cellulose nanofibers containing TiO₂ NPs. Moreover, to augment our nanofiber scaffolds with antibacterial activity, the in situ deposition approach of using Ag NPs was utilized with varied molar concentrations of 0.14, 0.42, and 0.71 M. The physicochemical properties of the nanofibers were identified by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) and contact angle meter studies. This demonstrated the presence of both TiO₂ and Ag NPs and complete deacetylation of nanofibers. The antibacterial efficiency of the nanofibers was scrutinized against Escherichia coli and Staphylococcus aureus, revealing proper in situ deposition of Ag NPs and confirming the nanofibers are antibacterial in nature. The biocompatibility of the scaffolds was accustomed using chicken embryo fibroblasts, which confirmed their potential role to be used as wound‐healing materials. Furthermore, the fabricated scaffolds were subjected to analysis in simulated body fluid at 37°C to induce mineralization for future osseous tissue integration. These results indicate that fabricated composite nanofiber scaffolds with multifunctional characteristics will have a highest potential as a future candidate for promoting new tissues artificially.

中文翻译：

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具有抗菌和生物活性的多功能纤维素/TiO2/Ag复合纳米纤维支架的制备，用于未来的组织工程应用。

在目前的工作中，探索了一种新的策略来制造纳米纤维支架，该支架由与二氧化钛 (TiO ₂ ) 和银 (Ag) 纳米颗粒 (NPs)同化的纤维素组成。相对于用于胶体溶液静电纺丝的聚合物浓度，将复合材料中TiO ₂ NPs的浓度调整为 1.0、1.5 和 2.0 重量％。使用 0.05 M NaOH 将制备的复合支架分配到碱性脱乙酰基团以去除乙酰基团，以生成含有 TiO _{2 的}纯纤维素纳米纤维NP。此外，为了增强我们的纳米纤维支架的抗菌活性，使用 Ag NPs 的原位沉积方法采用了 0.14、0.42 和 0.71 M 的不同摩尔浓度。 通过扫描电子显微镜 (SEM) 鉴定了纳米纤维的物理化学性质、透射电子显微镜 (TEM)、傅里叶变换红外 (FTIR) 和接触角仪研究。这证明了 TiO ₂和 Ag NPs的存在以及纳米纤维的完全脱乙酰化。对纳米纤维对大肠杆菌和金黄色葡萄球菌的抗菌效率进行了仔细检查，揭示了 Ag NPs 的适当原位沉积，并证实了纳米纤维在性质上具有抗菌性。支架的生物相容性已被用于鸡胚胎成纤维细胞，这证实了它们作为伤口愈合材料的潜在作用。此外，制造的支架在 37°C 的模拟体液中进行分析，以诱导矿化，以促进未来的骨组织整合。这些结果表明，具有多功能特性的复合纳米纤维支架作为未来人工促进新组织的候选者将具有最高的潜力。