Bio-Orthogonal AIEgen for Specific Discrimination and Elimination of Bacterial Pathogens via Metabolic Engineering,Chemistry of Materials

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Bio-Orthogonal AIEgen for Specific Discrimination and Elimination of Bacterial Pathogens via Metabolic Engineering
Chemistry of Materials ( IF 7.2 ) Pub Date : 2020-01-10 , DOI: 10.1021/acs.chemmater.9b04520
Min Wu ₁ , Guobin Qi ₁ , Xingang Liu ₁ , Yukun Duan ₁ , Jingjing Liu ₁ , Bin Liu ₁

Affiliation

Multiple bacterial infections caused by interpatient spread lead to complications in diagnosis and clinical management, seriously threatening human health. In this contribution, we design a bio-orthogonal fluorescence turn-on probe TPEPA for the discrimination and precise ablation of bacterial pathogens. The probe has good water solubility and negligible fluorescence in aqueous media. On clicking with the azide groups decorated on bacteria by metabolic engineering, it shows fluorescence turn-on with far-red/near-infrared emission. Taking advantage of the difference in bacterial structures, TPEPA can discriminate pathogens via selective imaging of metabolically decorated Gram-negative bacteria with Kdo-N₃ and Gram-positive bacteria with d-Ala-N₃, respectively, even in a mixture. Furthermore, TPEPA can effectively generate reactive oxygen species, which allows imaging, discrimination, and precise ablation functions to be integrated into one system to realize a selective antimicrobial effect.

中文翻译：

生物正交AIEgen通过代谢工程特异性区分和消除细菌病原体

由患者之间传播引起的多种细菌感染导致诊断和临床管理方面的并发症，严重威胁着人类健康。在这项贡献中，我们设计了一种生物正交荧光开启探针TPEPA，用于区分和精确消融细菌病原体。该探针在水性介质中具有良好的水溶性和可忽略的荧光。单击通过代谢工程修饰在细菌上的叠氮化物基团时，显示出具有远红/近红外发射的荧光开启。利用细菌结构的差异，TPEPA可以通过对带有Kdo-N ₃的新陈代谢的革兰氏阴性细菌和带有d的革兰氏阳性细菌的选择性成像来区分病原体。-Ala-N ₃甚至分别以混合物形式存在。此外，TPEPA可以有效生成活性氧，从而可以将成像，鉴别和精确的消融功能集成到一个系统中，从而实现选择性的抗菌效果。

更新日期：2020-01-10

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