Gestational diabetes mellitus (GDM) is a common clinical disease with complicated clinical process and harmful effects on pregnant women and fetus. The purpose of this study is to use the convenient urine samples in combination with glucose levels to detect or predict GDM. In this study, urine samples of non-pregnant women, normal pregnant women and GDM patients were collected. The peptides in urine were enriched by weak cationic exchange magnetic beads (MB-WCX) and analyzed by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). 46 polypeptide peaks with statistical difference (P < 0.01) were screened out by using Bioexplorer analysis software. The level of molecules with mass-to-charge ratio of 1079.2, 1290.6 and 1500.7 was higher in the GDM group than the other two groups. Through the analysis of differential molecules by liquid chromatography tandem mass spectrometry (LC-MS), the above molecules were identified as coagulation factor IX, TBC1 family member 5 isoform a [Homo sapiens] (TBC1D5a) and immunoglobulin kappa constant. The discovery of polypeptides provides the research basis for further primary screening and assistant diagnosis of GDM through urine samples.

中文翻译：

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妊娠糖尿病尿液中小分子肽的筛选和分析。

妊娠期糖尿病（GDM）是一种常见的临床疾病，其临床过程复杂且对孕妇和胎儿具有有害作用。这项研究的目的是将方便的尿液样本与葡萄糖水平结合使用，以检测或预测GDM。在这项研究中，收集了非孕妇，正常孕妇和GDM患者的尿液样本。尿液中的肽通过弱阳离子交换磁珠（MB-WCX）富集，并通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）进行分析。使用Bioexplorer分析软件筛选出具有统计学差异（P <0.01）的46个多肽峰。GDM组的质荷比为1079.2、1290.6和1500.7的分子水平高于其他两组。通过液相色谱串联质谱法（LC-MS）分析差异分子，上述分子被鉴定为凝血因子IX，TBC1家族成员5同工型[智人]（TBC1D5a）和免疫球蛋白κ常数。多肽的发现为进一步通过尿液样本对GDM进行初步筛查和辅助诊断提供了研究基础。