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The Arabidopsis UDP-glycosyltransferase75B1, conjugates abscisic acid and affects plant response to abiotic stresses.
Plant Molecular Biology ( IF 3.9 ) Pub Date : 2020-01-01 , DOI: 10.1007/s11103-019-00953-4
Ting-Ting Chen 1 , Fang-Fei Liu 1 , Dong-Wang Xiao 1 , Xiao-Yi Jiang 1 , Pan Li 1, 2 , Shu-Man Zhao 1 , Bing-Kai Hou 1 , Yan-Jie Li 1
Affiliation  

This study revealed that the Arabidopsis UGT75B1 plays an important role in modulating ABA activity by glycosylation when confronting stress environments. The cellular ABA content and activity can be tightly controlled in several ways, one of which is glycosylation by family 1 UDP-glycosyltransferases (UGTs). Previous analysis has shown UGT75B1 activity towards ABA in vitro. However, the biological role of UGT75B1 remains to be elucidated. Here, we characterized the function of UGT75B1 in abiotic stress responses via ABA glycosylation. GUS assay and qRT-PCR indicated that UGT75B1 is significantly upregulated by adverse conditions, such as osmotic stress, salinity and ABA. Overexpression of UGT75B1 in Arabidopsis leads to higher seed germination rates and seedling greening rates upon exposure to salt and osmotic stresses. In contrast, the big UGT75B1 overexpression plants are more sensitive under salt and osmotic stresses. Additionally, the UGT75B1 overexpression plants showed larger stomatal aperture and more water loss under drought condition, which can be explained by lower ABA levels examined in UGT75B1 OE plants in response to water deficit conditions. Consistently, UGT75B1 ectopic expression leads to downregulation of many ABA-responsive genes under stress conditions, including ABI3, ABI5 newly germinated seedlings and RD29A, KIN1, AIL1 in big plants. In summary, our results revealed that the Arabidopsis UGT75B1 plays an important role in coping with abiotic stresses via glycosylation of ABA.

中文翻译:

拟南芥 UDP-糖基转移酶 75B1 与脱落酸结合并影响植物对非生物胁迫的反应。

该研究表明,拟南芥 UGT75B1 在面临压力环境时通过糖基化调节 ABA 活性发挥重要作用。细胞 ABA 含量和活性可以通过多种方式进行严格控制,其中之一是家族 1 UDP-糖基转移酶 (UGT) 的糖基化。先前的分析已显示 UGT75B1 在体外对 ABA 的活性。然而,UGT75B1 的生物学作用仍有待阐明。在这里,我们通过 ABA 糖基化表征了 UGT75B1 在非生物应激反应中的功能。GUS 测定和 qRT-PCR 表明 UGT75B1 在渗透胁迫、盐度和 ABA 等不利条件下显着上调。UGT75B1 在拟南芥中的过表达导致暴露于盐和渗透胁迫时更高的种子发芽率和幼苗绿化率。相比之下,大的UGT75B1过表达植物在盐和渗透胁迫下更敏感。此外,UGT75B1 过表达植物在干旱条件下表现出更大的气孔孔径和更多的水分流失,这可以通过在 UGT75B1 OE 植物中检测到的较低的 ABA 水平来响应缺水条件来解释。一致地,UGT75B1异位表达导致许多ABA响应基因在胁迫条件下下调,包括大植物中的ABI3、ABI5新发芽幼苗和RD29A、KIN1、AIL1。总之,我们的研究结果表明,拟南芥 UGT75B1 通过 ABA 的糖基化在应对非生物胁迫中起重要作用。UGT75B1 过表达植物在干旱条件下表现出更大的气孔孔径和更多的水分流失,这可以通过在 UGT75B1 OE 植物中检测到的较低的 ABA 水平来响应缺水条件来解释。一致地,UGT75B1异位表达导致许多ABA响应基因在胁迫条件下下调,包括大植物中的ABI3、ABI5新发芽幼苗和RD29A、KIN1、AIL1。总之,我们的研究结果表明,拟南芥 UGT75B1 通过 ABA 的糖基化在应对非生物胁迫中起重要作用。UGT75B1 过表达植物在干旱条件下表现出更大的气孔孔径和更多的水分流失,这可以通过在 UGT75B1 OE 植物中检测到的较低的 ABA 水平来响应缺水条件来解释。一致地,UGT75B1异位表达导致许多ABA响应基因在胁迫条件下下调,包括大植物中的ABI3、ABI5新发芽幼苗和RD29A、KIN1、AIL1。总之,我们的研究结果表明,拟南芥 UGT75B1 通过 ABA 的糖基化在应对非生物胁迫中起重要作用。ABI5新发芽苗和大株RD29A、KIN1、AIL1。总之,我们的研究结果表明,拟南芥 UGT75B1 通过 ABA 的糖基化在应对非生物胁迫中起重要作用。ABI5新发芽苗和大株RD29A、KIN1、AIL1。总之,我们的研究结果表明,拟南芥 UGT75B1 通过 ABA 的糖基化在应对非生物胁迫中起重要作用。
更新日期:2020-01-01
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