mRNA display is a robust in vitro selection technique that allows the selection of peptides and proteins with desired functions from libraries of trillions of variants. mRNA display relies upon a covalent linkage between a protein and its encoding mRNA molecule; the power of the technique stems from the stability of this link, and the large degree of control over experimental conditions afforded to the researcher. This article describes the major advantages that make mRNA display the method of choice among comparable in vivo and in vitro methods, including cell-surface display, phage display, and ribosomal display. We also describe innovative techniques that harness mRNA display for directed evolution, protein engineering, and drug discovery.

中文翻译：

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肽和蛋白质的体外选择-mRNA展示的优势。

mRNA展示是一种强大的体外选择技术，可从数万亿个变体的文库中选择具有所需功能的肽和蛋白质。mRNA展示依赖于蛋白质与其编码的mRNA分子之间的共价键；该技术的力量源于这种联系的稳定性，并为研究人员提供了对实验条件的高度控制。本文介绍使mRNA展示可比的体内和体外方法中选择的方法的主要优点，包括细胞表面展示，噬菌体展示和核糖体展示。我们还描述了利用mRNA显示进行定向进化，蛋白质工程和药物发现的创新技术。