Curcumin (Cur) is widely used as an anti-inflammation agent and has anti-depression potential. Neuroinflammation mediated by Ca2+ channel activation is closely associated with the progression of post-stroke depression (PSD). In the current study, the role of P2X7 receptor (P2X7R) in the anti-PSD function of Cur was explored. Rats were subjected to middle cerebral artery occlusion (MCAO) surgery and chronic mild stress administration to induce PSD symptoms and then treated with Cur. The behaviors of rats were assessed with sucrose preference and forced swim tests. The accumulation of Ca2+ and the systemic inflammatory response in rats were detected. To determine the role of P2X7R in the anti-PSD function of curcumin, the PSD mice were further administrated with P2X7R agonist and antagonist. The administration of Cur attenuated behavior disorders associated with PSD. Moreover, the Ca2+ accumulation and the inflammatory response associated with PSD were also blocked by Cur. Cur also inhibited the activation of Ca2+ channel. The induced activity of P2X7R blocked the function of Cur by maintaining the symptoms of PSD in Cur-treated rats. Collectively, the anti-PSD function of Cur was dependent on the inhibition of P2X7R, which then deactivated Ca2+ channel-mediated inflammatory response associated with PSD progression.

中文翻译：

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姜黄素通过抑制 P2X7 受体的激活来改善小胶质细胞中的 Ca2+ 失调。


姜黄素 (Cur) 被广泛用作抗炎剂，并具有抗抑郁潜力。 Ca2+通道激活介导的神经炎症与中风后抑郁症（PSD）的进展密切相关。本研究探讨了P2X7受体（P2X7R）在Cur抗PSD功能中的作用。对大鼠进行大脑中动脉闭塞（MCAO）手术和慢性轻度应激给药以诱导 PSD 症状，然后用 Cur 治疗。通过蔗糖偏好和强迫游泳测试评估大鼠的行为。检测大鼠体内Ca2+蓄积及全身炎症反应。为了确定 P2X7R 在姜黄素的抗 PSD 功能中的作用，进一步给 PSD 小鼠施用 P2X7R 激动剂和拮抗剂。 Cur 的施用可减轻与 PSD 相关的行为障碍。此外，Cur 还可阻断与 PSD 相关的 Ca2+ 积累和炎症反应。 Cur 还抑制 Ca2+ 通道的激活。 P2X7R 诱导的活性通过维持 Cur 治疗大鼠的 PSD 症状来阻断 Cur 的功能。总的来说，Cur 的抗 PSD 功能依赖于对 P2X7R 的抑制，从而使 Ca2+ 通道介导的与 PSD 进展相关的炎症反应失活。