Curcumin (Cur) is widely used as an anti-inflammation agent and has anti-depression potential. Neuroinflammation mediated by Ca2+ channel activation is closely associated with the progression of post-stroke depression (PSD). In the current study, the role of P2X7 receptor (P2X7R) in the anti-PSD function of Cur was explored. Rats were subjected to middle cerebral artery occlusion (MCAO) surgery and chronic mild stress administration to induce PSD symptoms and then treated with Cur. The behaviors of rats were assessed with sucrose preference and forced swim tests. The accumulation of Ca2+ and the systemic inflammatory response in rats were detected. To determine the role of P2X7R in the anti-PSD function of curcumin, the PSD mice were further administrated with P2X7R agonist and antagonist. The administration of Cur attenuated behavior disorders associated with PSD. Moreover, the Ca2+ accumulation and the inflammatory response associated with PSD were also blocked by Cur. Cur also inhibited the activation of Ca2+ channel. The induced activity of P2X7R blocked the function of Cur by maintaining the symptoms of PSD in Cur-treated rats. Collectively, the anti-PSD function of Cur was dependent on the inhibition of P2X7R, which then deactivated Ca2+ channel-mediated inflammatory response associated with PSD progression.

中文翻译：

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姜黄素通过抑制P2X7受体的活化来改善小胶质细胞中的Ca2 +失调。

姜黄素（Cur）被广泛用作抗炎剂，并具有抗抑郁的潜力。由Ca2 +通道激活介导的神经炎症与中风后抑郁症（PSD）的进展密切相关。在当前的研究中，探讨了P2X7受体（P2X7R）在Cur的抗PSD功能中的作用。对大鼠进行大脑中动脉闭塞（MCAO）手术和慢性轻度应激管理，以诱发PSD症状，然后用Cur治疗。用蔗糖偏爱和强迫游泳试验评估大鼠的行为。检测大鼠体内Ca2 +的积累和全身炎症反应。为了确定P2X7R在姜黄素的抗PSD功能中的作用，将PSD小鼠进一步与P2X7R激动剂和拮抗剂一起给药。给予Cur减轻与PSD相关的行为障碍。此外，Cur也阻断了Ca2 +的蓄积和与PSD相关的炎症反应。Cur还抑制了Ca2 +通道的激活。P2X7R的诱导活性通过维持受Cur治疗的大鼠的PSD症状而阻断了Cur的功能。总的来说，Cur的抗PSD功能取决于对P2X7R的抑制作用，然后抑制P2X7R激活与PSD进展相关的Ca2 +通道介导的炎症反应。