Coelomic fluid contains a population of coelomocytes, enzymes, nutrients and kinds of molecules that could be essential for Apostichopus japonicus live. The coelom and polian vesicle are the main tissues that hold the most coelomic fluid in the animal, but whether there exists any immunological difference of the coelomic fluid from the two tissues remains unknown. In this study, we first extracted the coelomic fluid both from the coelom and polian vesicle, and compared their non-specific immune factors. It was found that the ACP and AKP activities in the polian vesicle were significantly higher than those in the coelom, but it was contrary for the SOD and CAT. Meanwhile, the expression levels of several immune-related genes including AjC3-2, AjMKK3/6, AjTLR3 and AjToll in the polian vesicle were significantly lower than those in the coelom. Besides, the early changes of non-specific immune factors were further monitored after eviscerated. During 7 days post evisceration, the immunoenzymes activities of ACP, AKP, SOD and CAT were decreased first and then recovered gradually in the coelomic fluid from the coelom. In the polian vesicle, the ACP and AKP activities showed a similar trend with the coelom, while the SOD and CAT activities showed a transitory increase during 2 h post evisceration (hpe) to 12 hpe. Moreover, the expression profiles of the immune-related genes in the coelom reached the peak at 3 days post evisceration (dpe), while their expression levels in the polian vesicle reached the peak at 7 dpe. All the results suggested that the immunocompetence of coelomic fluid differed in the coelom and polian vesicle, and thus may exert their respective immunological functions. It was likely that the respond speed in the coelom would be faster than that in the polian vesicle after evisceration. Our data will provide a basis for better understanding of the immune defense mechanism of A. japonicus.

中文翻译：

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刺突和小肠腔腔积液中非特异性免疫因子的差异，以及内脏去除后的早期反应。

腔积液中含有大量的内皮细胞，酶，营养物质和各种分子，这些分子可能是日本刺参活着的必不可少的部分。腔积液和囊是动物中拥有最多腔积液的主要组织，但是尚不清楚这两种组织中腔积液的免疫学差异。在这项研究中，我们首先从腔体和po囊中提取腔体液，并比较它们的非特异性免疫因子。结果发现，小囊泡中的ACP和AKP活性显着高于肠腔中的活性，但SOD和CAT却相反。同时，Poly囊泡中包括AjC3-2，AjMKK3 / 6，AjTLR3和AjToll在内的几种免疫相关基因的表达水平均显着低于结肠。此外，在内脏切除后进一步监测非特异性免疫因子的早期变化。内脏切除后的7天内，ACP，AKP，SOD和CAT的免疫酶活性先降低，然后在体液中从体腔逐渐恢复。在Poly囊泡中，ACP和AKP活性显示出与体腔相似的趋势，而SOD和CAT活性显示在拔除后2 h（hpe）到12 hpe之间有短暂的增加。此外，腔内免疫相关基因的表达谱在去内脏（dpe）后3天达到峰值，而它们在小囊泡中的表达水平在7dpe时达到峰值。所有结果表明，腔积液和多囊泡中腔积液的免疫能力不同，因此可能发挥各自的免疫功能。去除内脏后，体腔中的反应速度可能会比极化囊泡中的反应速度更快。我们的数据将为更好地了解日本刺槐的免疫防御机制提供基础。