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Nix is a male-determining factor in the Asian tiger mosquito Aedes albopictus.
Insect Biochemistry and Molecular Biology ( IF 3.8 ) Pub Date : 2019-12-31 , DOI: 10.1016/j.ibmb.2019.103311
Peiwen Liu 1 , Binbin Jin 1 , Xiaocong Li 1 , Yijie Zhao 1 , Jinbao Gu 1 , James K Biedler 2 , Zhijian Jake Tu 2 , Xiao-Guang Chen 1
Affiliation  

The initial signal that governs sex determination is highly variable among insects. A homolog of Nix, the male-determining factor in Aedes aegypti, was previously found in the Asian tiger mosquito Ae. albopictus. Here we show that the Ae. albopictus Nix (AalNix) is more complex in gene structure and splice isoforms than its Ae. aegypti homolog (AaeNix). AalNix shows a similar transcription profile compared to AaeNix. CRISPR/Cas9-mediated knockouts of AalNix in vivo and in the Ae. albopictus C6/36 cells lead to a shift of dsx and fru splicing towards the female isoforms. G0 knockout males showed feminization and deformities including feminized antennae, absence or partial absence of gonocoxites, gonostyli, testes and accessory glands, and the formation of ovaries. Despite ~70 MY of divergence, Nix functions as a conserved male-determining factor in the two most important arboviral vectors, namely Ae. aegypti and Ae. albopictus.

中文翻译:

Nix 是亚洲虎蚊白纹伊蚊的雄性决定因子。

控制性别决定的初始信号在昆虫之间变化很大。Nix 的同系物是埃及伊蚊的雄性决定因子,之前曾在亚洲虎蚊 Ae 中发现过。白纹伊蚊。在这里,我们表明 Ae。albopictus Nix (AalNix) 在基因结构和剪接亚型方面比其 Ae 更复杂。埃及伊蚊同系物 (AaeNix)。与 AaeNix 相比,AalNix 显示出相似的转录谱。体内和 Ae 中 CRISPR/Cas9 介导的 AalNix 敲除。白纹伊蚊 C6/36 细胞导致 dsx 和 fru 剪接向雌性亚型转变。G0 敲除雄性表现出女性化和畸形,包括女性化的触角、无或部分无性腺、生殖腺、睾丸和附属腺,以及卵巢的形成。尽管有大约 70 MY 的分歧,Nix 在两个最重要的虫媒病毒载体(即 Ae)中充当保守的雄性决定因子。埃及伊蚊和 Ae。白纹伊蚊。
更新日期:2020-01-01
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