In this perspective article, we reflect on the recent debate about the existence of human neurogenesis and discuss direct, and also indirect, support for the ongoing formation, and functional relevance, of new neurons in the adult and aged human hippocampus. To explain the discrepancies between several prominently published human studies, we discuss critical methodological aspects and highlight the importance of optimal tissue preservation and processing for histological examination. We further discuss novel approaches, like single-cell/nucleus sequencing and magnetic resonance spectroscopy, that will help advance the study of human neurogenesis to its fullest potential - understanding its contribution to human hippocampal functions and related disorders like depression and dementia.

中文翻译：

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成人神经发生，毕竟是人类（经典），优化的和未来的方法。

在这篇观点文章中，我们回顾了有关人类神经发生的最新争论，并讨论了对成人和老年人海马中新神经元的持续形成和功能相关性的直接和间接支持。为了解释几项著名的人体研究之间的差异，我们讨论了关键的方法论方面，并强调了最佳组织保存和处理对组织学检查的重要性。我们将进一步讨论新颖的方法，例如单细胞/核测序和磁共振波谱学，这将有助于最大程度地推动人类神经发生的研究-了解其对人类海马功能以及相关疾病（如抑郁症和痴呆症）的贡献。