Immune monitoring using mass cytometry and related high-dimensional imaging approaches.,Nature Reviews Rheumatology

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Immune monitoring using mass cytometry and related high-dimensional imaging approaches.
Nature Reviews Rheumatology ( IF 29.4 ) Pub Date : 2019-12-31 , DOI: 10.1038/s41584-019-0338-z
Felix J Hartmann ₁ , Sean C Bendall ₁

Affiliation

The cellular complexity and functional diversity of the human immune system necessitate the use of high-dimensional single-cell tools to uncover its role in multifaceted diseases such as rheumatic diseases, as well as other autoimmune and inflammatory disorders. Proteomic technologies that use elemental (heavy metal) reporter ions, such as mass cytometry (also known as CyTOF) and analogous high-dimensional imaging approaches (including multiplexed ion beam imaging (MIBI) and imaging mass cytometry (IMC)), have been developed from their low-dimensional counterparts, flow cytometry and immunohistochemistry, to meet this need. A growing number of studies have been published that use these technologies to identify functional biomarkers and therapeutic targets in rheumatic diseases, but the full potential of their application to rheumatic disease research has yet to be fulfilled. This Review introduces the underlying technologies for high-dimensional immune monitoring and discusses aspects necessary for their successful implementation, including study design principles, analytical tools and future developments for the field of rheumatology.

中文翻译：

使用质谱流式细胞术和相关高维成像方法进行免疫监测。

人类免疫系统的细胞复杂性和功能多样性需要使用高维单细胞工具来揭示其在风湿性疾病以及其他自身免疫性疾病和炎症性疾病等多方面疾病中的作用。已经开发出使用元素（重金属）报告离子的蛋白质组学技术，例如质谱流式细胞术（也称为 CyTOF）和类似的高维成像方法（包括多重离子束成像（MIBI）和成像质谱流式细胞术（IMC））流式细胞术和免疫组织化学等低维对应物可以满足这一需求。越来越多的研究已经发表，利用这些技术来识别风湿病的功能生物标志物和治疗靶点，但它们在风湿病研究中的应用潜力尚未得到充分发挥。本综述介绍了高维免疫监测的基础技术，并讨论了其成功实施所需的各个方面，包括研究设计原则、分析工具和风湿病学领域的未来发展。

更新日期：2019-12-31

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