Abstract This study presents essential oil composition of three Melaleuca species namely, Melaleuca bracteata F. Muell , Melaleuca fulgens R. Br. subsp. steedmanii and Melaleuca leucadendron (L.) L. collected from different regions of Pakistan. The chemical composition of essential oils was analyzed by GC-FID and GC–MS. Eugenol methyl ether was identified as a principal component in M. bracteata (82.3%) , M. fulgens (87.8%) and M. leucadendron (95.4%) oils. In vitro antibacterial studies were done by agar well diffusion and microdilution method and the tested essential oils exhibited bacteriostatic and bactericidal effects against the tested foodborne pathogens at 4–8 µg/ml. Time kill assay showed significant bactericidal effect of oils for four weeks. The antioxidant potential was assessed by free radical scavenging activity and reducing power assay. The oils showed strong antioxidant activity with approximately 89.0–89.5% inhibition of 2,2-diphenyl-1-picrylhydrazyl radical and ferric reducing power in the range of 1.94 ± 0.007–2.04 ± 0.04% at 100 µg/ml.

中文翻译：

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三种巴基斯坦白千层叶精油的化学成分、抗菌和抗氧化活性

摘要 本研究介绍了三种白千层的精油成分，即大白千层 F. Muell 、Melaleuca fulgens R. Br。亚种 从巴基斯坦不同地区收集的 steedmanii 和 Melaleuca leucadendron (L.) L.。通过 GC-FID 和 GC-MS 分析精油的化学成分。丁香酚甲基醚被鉴定为 M. bracteata (82.3%)、M. fulgens (87.8%) 和 M. leucadendron (95.4%) 油中的主要成分。通过琼脂孔扩散和微量稀释方法进行体外抗菌研究，测试的精油在 4–8 µg/ml 时对测试的食源性病原体表现出抑菌和杀菌作用。时间杀灭试验显示油具有显着的杀菌作用，持续 4 周。通过自由基清除活性和还原能力测定来评估抗氧化潜力。这些油显示出很强的抗氧化活性，对 2,2-二苯基-1-苦基肼自由基的抑制率约为 89.0-89.5%，在 100 µg/ml 时，铁的还原能力在 1.94 ± 0.007-2.04 ± 0.04% 的范围内。