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Development of high-speed ion conductance microscopy
Review of Scientific instruments ( IF 1.587 ) Pub Date : 2019-12-23 , DOI: 10.1063/1.5118360
Shinji Watanabe, Satoko Kitazawa, Linhao Sun, Noriyuki Kodera, Toshio Ando

Scanning ion conductance microscopy (SICM) can image the surface topography of specimens in ionic solutions without mechanical probe–sample contact. This unique capability is advantageous for imaging fragile biological samples but its highest possible imaging rate is far lower than the level desired in biological studies. Here, we present the development of high-speed SICM. The fast imaging capability is attained by a fast Z-scanner with active vibration control and pipette probes with enhanced ion conductance. By the former, the delay of probe Z-positioning is minimized to sub-10 µs, while its maximum stroke is secured at 6 μm. The enhanced ion conductance lowers a noise floor in ion current detection, increasing the detection bandwidth up to 100 kHz. Thus, temporal resolution 100-fold higher than that of conventional systems is achieved, together with spatial resolution around 20 nm.
更新日期:2019-12-31

 

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