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A Ribose-Scavenging System Confers Colonization Fitness on the Human Gut Symbiont Bacteroides thetaiotaomicron in a Diet-Specific Manner.
Cell Host & Microbe ( IF 20.6 ) Pub Date : 2019-12-31 , DOI: 10.1016/j.chom.2019.11.009 Robert W P Glowacki 1 , Nicholas A Pudlo 1 , Yunus Tuncil 2 , Ana S Luis 1 , Peter Sajjakulnukit 3 , Anton I Terekhov 2 , Costas A Lyssiotis 3 , Bruce R Hamaker 2 , Eric C Martens 1
Cell Host & Microbe ( IF 20.6 ) Pub Date : 2019-12-31 , DOI: 10.1016/j.chom.2019.11.009 Robert W P Glowacki 1 , Nicholas A Pudlo 1 , Yunus Tuncil 2 , Ana S Luis 1 , Peter Sajjakulnukit 3 , Anton I Terekhov 2 , Costas A Lyssiotis 3 , Bruce R Hamaker 2 , Eric C Martens 1
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Efficient nutrient acquisition in the human gut is essential for microbial persistence. Although polysaccharides have been well-studied nutrients for the gut microbiome, other resources such as nucleic acids and nucleosides are less studied. We describe several ribose-utilization systems (RUSs) that are broadly represented in Bacteroidetes and appear to have diversified to access ribose from a variety of substrates. One Bacteroides thetaiotaomicron RUS variant is critical for competitive gut colonization in a diet-specific fashion. We used molecular genetics to probe the required functions of the system and the nature of the nutrient source(s) underlying this phenotype. Two RUS-encoded ribokinases were the only components required for this effect, presumably because they generate ribose-phosphate derivatives from products of an unlinked but essential nucleoside phosphorylase. Our results underscore the extensive mechanisms that gut symbionts have evolved to access nutrients and the potential for unexpected dependencies among systems that mediate colonization and persistence.
中文翻译：
核糖清除系统以特定饮食的方式赋予人类肠道共生细菌拟杆菌Thetaiotaomicron一定的定殖适应性。
有效吸收人体肠道中的营养对于微生物的持久性至关重要。尽管多糖已被广泛研究为肠道微生物组的营养素，但对诸如核酸和核苷等其他资源的研究却很少。我们描述了在拟杆菌中广泛代表的几种核糖利用系统（RUSs），并且似乎已经多样化地从各种底物中获取核糖。一种拟杆菌化的thetaiotaomicron RUS变体以饮食特定的方式对于竞争性肠道定植至关重要。我们使用分子遗传学来探究系统所需的功能以及该表型基础的营养来源的性质。两种RUS编码的核糖激酶是实现此效果所需的唯一组件，大概是因为它们从未连接但必不可少的核苷磷酸化酶的产物中产生核糖磷酸酯衍生物。我们的研究结果强调了肠道共生体已经发展出获取营养的广泛机制，并且在介导殖民化和持久性的系统之间可能存在意料之外的依赖性。
更新日期：2019-12-31
中文翻译：
核糖清除系统以特定饮食的方式赋予人类肠道共生细菌拟杆菌Thetaiotaomicron一定的定殖适应性。
有效吸收人体肠道中的营养对于微生物的持久性至关重要。尽管多糖已被广泛研究为肠道微生物组的营养素，但对诸如核酸和核苷等其他资源的研究却很少。我们描述了在拟杆菌中广泛代表的几种核糖利用系统（RUSs），并且似乎已经多样化地从各种底物中获取核糖。一种拟杆菌化的thetaiotaomicron RUS变体以饮食特定的方式对于竞争性肠道定植至关重要。我们使用分子遗传学来探究系统所需的功能以及该表型基础的营养来源的性质。两种RUS编码的核糖激酶是实现此效果所需的唯一组件，大概是因为它们从未连接但必不可少的核苷磷酸化酶的产物中产生核糖磷酸酯衍生物。我们的研究结果强调了肠道共生体已经发展出获取营养的广泛机制，并且在介导殖民化和持久性的系统之间可能存在意料之外的依赖性。
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