Silica particles with various pore sizes were modified with poly(acrylamide) via surface-initiated atom-transfer radical polymerization (SI-ATRP) under different reaction conditions. Twenty different columns were prepared and characterized according to a test method for hydrophilic interaction chromatography (HILIC) columns. Hydrophilic retention by the SI-ATRP columns was much higher than that of poly(acrylamide) columns prepared via free-radical polymerization and many commercially available HILIC columns. The SI-ATRP columns displayed greater selectivity for -OH groups than any of the HILIC columns based on their α(U/2dU) values. SI-ATRP functionalization was used to increase the polymer chain density on the silica particles, which suggested a brush-type morphology, and improved hydrophilic selectivity. This indicated that hydrophilic retention and selectivity could be controlled by adjusting the morphology of the organic stationary phase. This stationary phase design strategy was validated experimentally by the effective separation of highly hydrophilic analytes. The findings of this study will greatly contribute to the creation of better separation media.

中文翻译：

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二氧化硅颗粒上的聚合物结构与亲水相互作用色谱法相应色谱柱的分离特性之间的关系。

在不同的反应条件下，通过表面引发的原子转移自由基聚合（SI-ATRP），用聚丙烯酰胺改性了各种孔径的二氧化硅颗粒。制备了二十种不同的色谱柱，并根据亲水相互作用色谱（HILIC）色谱柱的测试方法进行了表征。SI-ATRP色谱柱的亲水保留率远远高于通过自由基聚合和许多市售HILIC色谱柱制备的聚丙烯酰胺色谱柱。基于SI-ATRP色谱柱的α（U / 2dU）值，它们对-OH基的选择性高于任何HILIC色谱柱。SI-ATRP功能化被用来增加二氧化硅颗粒上的聚合物链密度，这表明了刷状形态，并提高了亲水选择性。这表明可以通过调节有机固定相的形态来控制亲水性保留和选择性。该固定相设计策略已通过有效分离高度亲水的分析物进行了实验验证。这项研究的发现将大大有助于创建更好的分离介质。