Tracheal stenosis is a rare but life-threatening disease. Primary clinical procedures for treating this disease are limited if the region requiring repair is long or complex. This study is the first of its kind to fabricate bioprinted tracheal constructs with separate cartilage and smooth muscle regions using polycaprolactone (PCL) and human mesenchymal stem cell (hMSC)-laden hydrogels. Our final bioprinted trachea showed comparable elastic modulus and yield stress compared to native tracheal tissue. In addition, both cartilage and smooth muscle formation were observed in the desired regions of our bioprinted trachea through immunohistochemistry and western blot after two weeks of in vitro culture. This study demonstrates a novel approach to manufacture tissue engineered trachea with mechanical and biological properties similar to native trachea, which represents a step closer to overcoming the clinical challenges of treating tracheal stenosis.

中文翻译：

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具有患者匹配设计，机械和生物学特性的生物印制气管构造。

气管狭窄是一种罕见但危及生命的疾病。如果需要修复的区域较长或较复杂，则治疗该疾病的主要临床程序将受到限制。这项研究是首次使用聚己内酯（PCL）和人间充质干细胞（hMSC）载有水凝胶来制造具有独立软骨和平滑肌区域的生物打印气管构建体。与天然气管组织相比，我们最终的生物打印气管显示出可比的弹性模量和屈服应力。此外，在体外培养两周后，通过免疫组织化学和蛋白质印迹，在我们的生物印制气管的所需区域中观察到了软骨和平滑肌的形成。这项研究展示了一种新颖的方法来制造组织工程气管，其机械和生物学特性与天然气管相似，