Pathogenic Bacteria Target Plant Plasmodesmata to Colonize and Invade Surrounding Tissues.,The Plant Cell

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Pathogenic Bacteria Target Plant Plasmodesmata to Colonize and Invade Surrounding Tissues.
The Plant Cell ( IF 10.0 ) Pub Date : 2019-12-30 , DOI: 10.1105/tpc.19.00707
Kyaw Aung _{1,

2} , Panya Kim ₃ , Zhongpeng Li ₂ , Anna Joe ₃ , Brian Kvitko ₄ , James R Alfano _{3,

5} , Sheng Yang He _{1,

6,

7,

8}

Affiliation

A hallmark of multicellular organisms is their ability to maintain physiological homeostasis by communicating among cells, tissues, and organs. In plants, intercellular communication is largely dependent on plasmodesmata (PD), which are membrane-lined channels connecting adjacent plant cells. Upon immune stimulation, plants close PD as part of their immune responses. Here, we show that the bacterial pathogen Pseudomonas syringae deploys an effector protein, HopO1-1, that modulates PD function. HopO1-1 is required for P. syringae to spread locally to neighboring tissues during infection. Expression of HopO1-1 in Arabidopsis (Arabidopsis thaliana) increases the distance of PD-dependent molecular flux between neighboring plant cells. Being a putative ribosyltransferase, the catalytic activity of HopO1-1 is required for regulation of PD. HopO1-1 physically interacts with and destabilizes the plant PD-located protein PDLP7 and possibly PDLP5. Both PDLPs are involved in bacterial immunity. Our findings reveal that a pathogenic bacterium utilizes an effector to manipulate PD-mediated host intercellular communication for maximizing the spread of bacterial infection.

中文翻译：

病原细菌以植物胞间连丝为目标定殖并侵入周围组织。

多细胞生物的一个标志是它们能够通过细胞、组织和器官之间的交流来维持生理稳态。在植物中，细胞间通讯很大程度上依赖于胞间连丝（PD），胞间连丝是连接相邻植物细胞的膜内通道。在受到免疫刺激后，植物会关闭 PD 作为其免疫反应的一部分。在这里，我们发现细菌病原体丁香假单胞菌部署了一种效应蛋白 HopO1-1，可以调节 PD 功能。在感染过程中，丁香假单胞菌需要 HopO1-1 才能局部传播到邻近组织。 HopO1-1 在拟南芥 (Arabidopsis thaliana) 中的表达增加了相邻植物细胞之间 PD 依赖性分子通量的距离。作为一种假定的核糖基转移酶，HopO1-1 的催化活性是 PD 调节所必需的。 HopO1-1 与植物 PD 定位蛋白 PDLP7 和可能的 PDLP5 发生物理相互作用并使其不稳定。两种 PDLP 都参与细菌免疫。我们的研究结果表明，致病细菌利用效应器来操纵 PD 介导的宿主细胞间通讯，以最大限度地扩大细菌感染的传播。

更新日期：2020-03-03

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