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Physical and biological effects of paclitaxel encapsulation on disteraroylphosphatidylethanolamine-polyethyleneglycol polymeric micelles.
Colloids and Surfaces B: Biointerfaces ( IF 5.4 ) Pub Date : 2019-12-31 , DOI: 10.1016/j.colsurfb.2019.110760 Caroline Mari Ramos Oda 1 , Antônio Augusto Malfatti-Gasperini 2 , Angelo Malachias 3 , Gwenaelle Pound-Lana 4 , Vanessa Carla Furtado Mosqueira 4 , Renata Salgado Fernandes 1 , Mônica Cristina de Oliveira 1 , André Luis Branco de Barros 5 , Elaine Amaral Leite 1
Colloids and Surfaces B: Biointerfaces ( IF 5.4 ) Pub Date : 2019-12-31 , DOI: 10.1016/j.colsurfb.2019.110760 Caroline Mari Ramos Oda 1 , Antônio Augusto Malfatti-Gasperini 2 , Angelo Malachias 3 , Gwenaelle Pound-Lana 4 , Vanessa Carla Furtado Mosqueira 4 , Renata Salgado Fernandes 1 , Mônica Cristina de Oliveira 1 , André Luis Branco de Barros 5 , Elaine Amaral Leite 1
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Simple size observations of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000] (DSPE-mPEG2000) polymeric micelles (PM) with different compositions including or not paclitaxel (PTX) are unable to evidence changes on the nanocarrier structure. In such system a detailed characterization using highly sensitive techniques such as X-ray scattering and asymmetric flow field flow fractionation coupled to multi-angle laser light scattering and dynamic light scattering (AF4-MALS-DLS) is mandatory to observe effects that take place by the addition of PTX and/or more lipid-polymer at PM, leading to complex changes on the structure of micelles, as well as in their supramolecular organization. SAXS and AF4-MALS-DLS suggested that PM can be found in the medium separately and highly organized, forming clusters of PM in the latter case. SAXS fitted parameters showed that adding the drug does not change the average PM size since the increase in core radius is compensated by the decrease in shell radius. SAXS observations indicate that PEG conformation takes place, changing from brush to mushroom depending on the PM composition. These findings directly reflect in in vivo studies of blood clearance that showed a longer circulation time of blank PM when compared to PM containing PTX.
中文翻译：
紫杉醇包封对二硬脂酰磷脂酰乙醇胺-聚乙二醇聚合物胶束的物理和生物学影响。
无法简单地观察具有不同组成（包括是否包含紫杉醇（PTX））的1,2-二硬脂酰基-sn-甘油-3-磷酸乙醇胺-N- [甲氧基（聚乙二醇）-2000]（DSPE-mPEG2000）聚合物胶束（PM）的大小证明纳米载体结构发生了变化。在这样的系统中，必须使用高灵敏度技术（例如X射线散射和不对称流场流动分离，再加上多角度激光散射和动态光散射（AF4-MALS-DLS））进行详细表征，才能观察到由在PM处添加PTX和/或更多脂质聚合物，导致胶束结构及其超分子组织发生复杂变化。SAXS和AF4-MALS-DLS建议，PM可以在介质中单独发现并且组织良好，在后一种情况下形成PM簇。SAXS拟合参数显示，添加药物不会改变平均PM大小，因为药心半径的增加被壳半径的减少所补偿。SAXS的观察结果表明，发生了PEG构象，具体取决于PM组成，从刷子变为蘑菇。这些发现直接反映了体内血液清除研究，与含PTX的PM相比，空白PM的循环时间更长。
更新日期：2019-12-31
中文翻译：
紫杉醇包封对二硬脂酰磷脂酰乙醇胺-聚乙二醇聚合物胶束的物理和生物学影响。
无法简单地观察具有不同组成（包括是否包含紫杉醇（PTX））的1,2-二硬脂酰基-sn-甘油-3-磷酸乙醇胺-N- [甲氧基（聚乙二醇）-2000]（DSPE-mPEG2000）聚合物胶束（PM）的大小证明纳米载体结构发生了变化。在这样的系统中，必须使用高灵敏度技术（例如X射线散射和不对称流场流动分离，再加上多角度激光散射和动态光散射（AF4-MALS-DLS））进行详细表征，才能观察到由在PM处添加PTX和/或更多脂质聚合物，导致胶束结构及其超分子组织发生复杂变化。SAXS和AF4-MALS-DLS建议，PM可以在介质中单独发现并且组织良好，在后一种情况下形成PM簇。SAXS拟合参数显示，添加药物不会改变平均PM大小，因为药心半径的增加被壳半径的减少所补偿。SAXS的观察结果表明，发生了PEG构象，具体取决于PM组成，从刷子变为蘑菇。这些发现直接反映了体内血液清除研究，与含PTX的PM相比，空白PM的循环时间更长。
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