Designation of chitosan nano-vaccine based on MxiH antigen of Shigella flexneri with increased immunization capacity.,Carbohydrate Polymers

当前位置： X-MOL 学术 › Carbohydr. Polym. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Designation of chitosan nano-vaccine based on MxiH antigen of Shigella flexneri with increased immunization capacity.
Carbohydrate Polymers ( IF 10.7 ) Pub Date : 2019-12-31 , DOI: 10.1016/j.carbpol.2019.115813
Farhad Gilavand ₁ , Abdolrazagh Marzban ₂ , Gholamhossein Ebrahimipour ₁ , Neda Soleimani ₁ , Mehdi Goudarzi ₃

Affiliation

Shigella flexneri is a gram-negative pathogen that causes shigellosis in humans and primates. MxiH antigen is known as one of the invasive factors in most Gram-negative bacteria consisting of a needle-like structure in the main backbone of the type 3 secretory system. Recombinant MxiH antigen was produced by E. coli BL21 and purified antigen was loaded into chitosan nanoparticles (CS-MxiH). After 20thand 55th of intranasal vaccinations, the titers of IgG, IgA, IL-4, and IFN-γ were evaluated. The results indicated the successful synthesis of CS nanoparticles followed by the effective loading of MxiH antigen. The results of animal experiments showed that the intranasal administration of CS-MxiH increased IgG and IgA compared to control groups. Increased levels of IL-4 and IFN-γ in groups immunized with CS-MxiH are probably due to the activation of plasmacytoid and myeloid cells presenting antigen in nasal epithelial mucosa and stimulating B cells.

中文翻译：

具有增强的免疫能力的基于志贺氏志贺氏菌的MxiH抗原的壳聚糖纳米疫苗的设计。

弗氏志贺氏菌是革兰氏阴性病原体，可在人类和灵长类动物中引起志贺菌病。MxiH抗原是大多数革兰氏阴性细菌中的一种侵入性因子，由3型分泌系统主要骨架中的针状结构组成。重组MxiH抗原由大肠杆菌BL21产生，纯化的抗原上样至壳聚糖纳米颗粒（CS-MxiH）中。鼻内接种20和55次后，评估IgG，IgA，IL-4和IFN-γ的效价。结果表明，成功合成了CS纳米颗粒，然后有效负载了MxiH抗原。动物实验结果表明，与对照组相比，鼻腔内给予CS-MxiH可增加IgG和IgA。

更新日期：2019-12-31

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11