Toehold Length of Target ssDNA Affects Its Reaction-Diffusion Behavior in DNA-Responsive DNA-co-Acrylamide Hydrogels.,Biomacromolecules

当前位置： X-MOL 学术 › Biomacromolecules › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Toehold Length of Target ssDNA Affects Its Reaction-Diffusion Behavior in DNA-Responsive DNA-co-Acrylamide Hydrogels.
Biomacromolecules ( IF 5.5 ) Pub Date : 2020-01-13 , DOI: 10.1021/acs.biomac.9b01515
Eleonóra Parelius Jonášová ₁ , Astrid Bjørkøy ₁ , Bjørn Torger Stokke ₁

Affiliation

In the present study, we expand on the understanding of hydrogels with embedded deoxyribonucleic acid (DNA) cross-links, from the overall swelling to characterization of processes that precede the swelling. The hydrogels respond to target DNA strands because of a toehold-mediated strand displacement reaction in which the target strand binds to and opens the dsDNA cross-link. The spatiotemporal evolution of the diffusing target ssDNA was determined using confocal laser scanning microscopy (CLSM). The concentration profiles revealed diverse partitioning of the target DNA inside the hydrogel as compared with the immersing solution: excluding a nonbinding DNA, while accumulating a binding target. The data show that a longer toehold results in faster cross-link opening but reduced diffusion of the target, thus resulting in only a moderate increase in the overall swelling rate. The parameters obtained by fitting the data using a reaction-diffusion model were discussed in view of the molecular parameters of the target ssDNA and hydrogels.

中文翻译：

目标ssDNA的前额长度会影响其在DNA响应型DNA-co-丙烯酰胺水凝胶中的反应扩散行为。

在本研究中，我们从整体溶胀到溶胀之前的过程表征，扩展了对具有嵌入式脱氧核糖核酸（DNA）交联的水凝胶的了解。由于脚趾介导的链置换反应，水凝胶对目标DNA链作出反应，其中目标链结合并打开dsDNA交联。使用共聚焦激光扫描显微镜（CLSM）确定了扩散目标ssDNA的时空演化。浓度曲线显示，与浸入溶液相比，目标DNA在水凝胶内部的分配方式多样：排除非结合DNA，同时积累结合靶。数据显示，更长的鞋头位置会导致更快的交联打开，但会减少目标的扩散，因此仅导致总体溶胀率适度增加。鉴于目标ssDNA和水凝胶的分子参数，讨论了通过使用反应扩散模型拟合数据而获得的参数。

更新日期：2019-12-30

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11