Facile Preparation of Hydrophilic Mesoporous Metal-Organic Framework via Synergistic Etching and Surface Functionalization for Glycopeptides Analysis.,Analytical Chemistry

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Facile Preparation of Hydrophilic Mesoporous Metal-Organic Framework via Synergistic Etching and Surface Functionalization for Glycopeptides Analysis.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-01-09 , DOI: 10.1021/acs.analchem.9b04236
Chenlu Pu ₁ , Hongli Zhao ₁ , Yayun Hong ₁ , Qiliang Zhan ₁ , Minbo Lan _{1,

2}

Affiliation

In view of the size and hydrophilicity of glycopeptides, materials having suitable channels (size-exclusion) and strong hydrophilic surface (hydrophilic interaction) are preferred to enrich the glycopeptides in biological samples. Metal-organic frameworks (MOFs) are good candidates. However, their smaller microporous channels and low chemical stability have limited the application. Herein, a facile strategy was established to construct hydrophilic mesoporous MOF via synergistic etching and surface functionalization by using phytic acid (PA). Besides, polyvinylpyrrolidone (PVP) was added during MOF synthesis to enhance the water stability of the MOF. Owing to the expanded hydrophilic mesoporous channels, the PA-modified Ce-MOF effectively and selectively captured 422 glycopeptides from 155 glycoproteins in tryptic digests of human serum (2 μL). The present work sheds light on the easy fabrication of hydrophilic mesoporous materials, and this established material holds unique advantages for glycopeptides analysis in biological samples.

中文翻译：

通过协同蚀刻和表面功能化来制备糖基介导的亲水介孔金属有机骨架。

考虑到糖肽的大小和亲水性，优选具有合适通道（尺寸排阻）和强亲水表面（亲水相互作用）的材料以富集生物样品中的糖肽。金属有机框架（MOF）是不错的选择。但是，它们较小的微孔通道和较低的化学稳定性限制了其应用。本文中，建立了一种简便的策略以通过使用植酸（PA）的协同蚀刻和表面功能化来构建亲水中孔MOF。此外，在MOF合成过程中添加了聚乙烯吡咯烷酮（PVP），以增强MOF的水稳定性。由于扩大了亲水性介孔通道，PA修饰的Ce-MOF有效地并选择性地从人血清胰蛋白酶消化物中的155种糖蛋白中捕获了422种糖肽（2μL）。

更新日期：2020-01-10

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