Tailored Suits Fit Better: Customized Protein Crystallization Screens,Crystal Growth & Design

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Tailored Suits Fit Better: Customized Protein Crystallization Screens
Crystal Growth & Design ( IF 3.2 ) Pub Date : 2020-01-13 , DOI: 10.1021/acs.cgd.9b01328
Georg Mlynek ₁ , Julius Kostan ₁ , Sarah Leeb ₁ , Kristina Djinović-Carugo _{1,

2}

Affiliation

The major bottleneck in macromolecular crystallography is the production of well-diffracting crystals. To increase the crystallization success, we developed a crystallization strategy customizable for each protein and tested it on selected model and in-house non-yet-crystallized proteins. The underlying concept is to use in crystallization cocktails compounds that increase the thermal stability of the protein. This customized strategy yielded not only twice as many crystal hits as the standard approach but also crystal hits for two additional proteins compared to the benchmark workflow. The inherently simple design and the modular and flexible nature of the platform makes it easy to modify, further develop, and optimize individual steps. The information gained can also be used to increase the monodispersity and stability of proteins, improving in this way their amenability for biochemical studies and eventually derive a possible function of not yet annotated proteins.

中文翻译：

量身定制的服装更合身：定制的蛋白质结晶筛选

大分子晶体学的主要瓶颈是产生高衍射晶体。为了增加结晶成功率，我们开发了可针对每种蛋白质定制的结晶策略，并在选定的模型和内部未结晶的蛋白质上对其进行了测试。基本概念是在结晶混合物中使用可增加蛋白质热稳定性的化合物。与基准工作流程相比，这种定制策略不仅产生的晶体命中率是标准方法的两倍，而且还产生了另外两种蛋白质的晶体命中率。平台固有的简单设计以及模块化和灵活的特性使它易于修改，进一步开发和优化各个步骤。获得的信息还可以用于增加蛋白质的单分散性和稳定性，

更新日期：2020-01-14

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