BACKGROUND Peach (Prunus persica L.) is a diploid species and model plant of the Rosaceae family. In the past decade, significant progress has been made in peach genetic research via DNA markers, but the number of these markers remains limited. RESULTS In this study, we performed a genome-wide DNA markers detection based on sequencing data of six distantly related peach accessions. A total of 650,693~1,053,547 single nucleotide polymorphisms (SNPs), 114,227~178,968 small insertion/deletions (InDels), 8386~12,298 structure variants (SVs), 2111~2581 copy number variants (CNVs) and 229,357~346,940 simple sequence repeats (SSRs) were detected and annotated. To demonstrate the application of DNA markers, 944 SNPs were filtered for association study of fruit ripening time and 15 highly polymorphic SSRs were selected to analyze the genetic relationship among 221 accessions. CONCLUSIONS The results showed that the use of high-throughput sequencing to develop DNA markers is fast and effective. Comprehensive identification of DNA markers, including SVs and SSRs, would be of benefit to genetic diversity evaluation, genetic mapping, and molecular breeding of peach.

中文翻译：

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桃子全基因组变异的检测和应用，用于关联和遗传关系分析。

背景技术桃（Prunus persica L.）是蔷薇科的二倍体物种和模型植物。在过去的十年中，通过DNA标记在桃子遗传研究方面取得了重大进展，但是这些标记的数量仍然有限。结果在这项研究中，我们基于六个远亲桃子的测序数据进行了全基因组DNA标记检测。总共650,693〜1,053,547个单核苷酸多态性（SNP），114,227〜178,968个小插入/缺失（InDels），8386〜12,298个结构变体（SV），2111〜2581个拷贝数变体（CNV）和229,357〜346,940个简单序列重复（ SSR）被检测到并添加了注释。为了演示DNA标记的应用，筛选出944个SNP用于果实成熟时间的关联研究，选择了15个高度多态的SSR，分析了221个材料之间的遗传关系。结论结果表明，使用高通量测序开发DNA标记是快速有效的。全面鉴定包括SV和SSR在内的DNA标记，将有助于桃的遗传多样性评估，遗传作图和分子育种。