Intravital imaging, the direct visualization of cells and tissues within a living animal, is a technique that has been employed for the better part of a century. The advent of confocal and multiphoton microscopy has dramatically improved the power of intravital imaging, making it possible to obtain optical sections of tissues non-destructively. This review discusses the various techniques used for intravital imaging, describes how intravital imaging provides information about cellular and tissue dynamics not possible to be garnered by other techniques, and details several ways in which intravital imaging is making a direct impact on the clinical care of patients. © 2019 International Society for Advancement of Cytometry.

中文翻译：

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用于生物医学和临床研究的活体成像技术。

活体成像，即活体动物体内细胞和组织的直接可视化，是一种已经使用了一个世纪的大部分时间的技术。共聚焦和多光子显微镜的出现极大地提高了活体成像的能力，使得非破坏性地获得组织的光学切片成为可能。本综述讨论了用于活体成像的各种技术，描述了活体成像如何提供其他技术无法获得的有关细胞和组织动力学的信息，并详细说明活体成像对患者临床护理产生直接影响的几种方式. © 2019 国际细胞计量学促进会。