Sunn pest or Sunn bug, Eurygaster integriceps Put., salivary gland proteases are responsible for the deterioration of wheat flour quality during dough mixing, resulting from gluten hydrolysis. These proteases are highly heterogeneous and show low sensitivity to most types of proteinaceous inhibitors, meaning that such inhibitors cannot be used to prevent gluten damage. The present study describes the generation of a specific peptide antibody, raised against the active center of the recombinant gluten‐hydrolyzing protease (GHP3). The recombinant protein, encoding two repeats of the GHP3 sequence element involved in forming the S4 pocket and binding of substrate at position P4, was designed and expressed in Escherichia coli. The antibodies raised to this recombinant protein showed inhibitory activity against the GHP3 protease. The results indicate that it is possible to design specific antibodies to inhibit wheat‐bug gluten‐hydrolyzing proteases.

中文翻译：

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针对Sunn bug（Eurygaster integriceps Put。）重组蛋白酶rGHP3p2产生的抗体可以抑制面筋水解活性。

唾液腺蛋白酶是虫或Sun虫（Eurygaster integriceps Put。），唾液腺蛋白酶是造成面筋水解过程中小麦粉品质下降的原因。这些蛋白酶是高度异质的，并且对大多数类型的蛋白质抑制剂显示出较低的敏感性，这意味着此类抑制剂不能用于预防面筋损伤。本研究描述了针对重组谷蛋白水解蛋白酶（GHP3）活性中心的特异性肽抗体的产生。在大肠杆菌中设计并表达了重组蛋白，该蛋白编码参与形成S4口袋和底物在P4位置结合的GHP3序列元件的两个重复序列。。针对该重组蛋白的抗体显示出对GHP3蛋白酶的抑制活性。结果表明，可以设计特异性抗体来抑制小麦虫麸质水解蛋白酶。