The conjugations of ZnONPS with a biological surface-active substance, folic acid (FA), may achieve more biological activity and medical applications. Therefore, the current research was aimed to synthesis the ZnONPS then conjugated with FA by a facile chemical method. The morphological studies for free ZnONPS and ZnONPS@FA was performed using the (TEM), (SEM), (XRD), (EDX) and Zeta-potential. The spectral analyses were followed by UV–vis measurements and Photoluminescence (PL). The structural composition and conjugation strategies between ZnONPS and FA were observed by (FT-IR). The thermal stability was investigated using Thermo gravimetrical analysis (TGA). The antimicrobial efficacy and the minimum inhibitory concentration (MIC) were examined against 10 human pathogens. The NPS was investigated for photocatalytic degradation of methylene blue dye (MB). The antioxidant activity was detected. The results were showed nanocrystalline wurtzite possess quasi spherical NPS with size 16 nm (λ_max = 373 nm, band gab 3.32 eV) after loading FA the sized increased by 2 nm with d spacing 0.3 nm. As FT-IR findings the FA and ZnONPS were linked via an amide bonding. The photo-degradation for MB after 180 min reached 82% and 85% for ZnONPS and ZnONPS@FA, respectively. The antioxidant activity for nanocomposite was enhanced as well as the antimicrobial for E. faecalis and others more than free NPS. The results of MIC and MBC values were ranged from16 to 256 μg mL⁻¹ and 16 to 512 μg mL⁻¹, respectively. In conclusion, we can conclude that incorporation of FA to NPS was enhanced its antioxidant and antimicrobial potential leading it to be promising in many biomedical and food packaging applications.

ZnONPS与生物表面活性物质叶酸（FA）的共轭可实现更多的生物活性和医学应用。因此，目前的研究旨在通过一种简便的化学方法合成然后与FA共轭的ZnONPS。使用（TEM），（SEM），（XRD），（EDX）和Zeta电位对游离ZnONPS和ZnONPS @ FA进行了形态学研究。光谱分析之后是紫外可见测量和光致发光（PL）。用（FT-IR）观察了ZnONPS和FA之间的结构组成和结合策略。使用热重量分析（TGA）研究了热稳定性。检查了对10种人类病原体的抗菌功效和最低抑菌浓度（MIC）。研究了NPS对亚甲基蓝染料（MB）的光催化降解。检测到抗氧化活性。结果表明，纳米晶纤锌矿具有准球形NPS，尺寸为16 nm（λ_max = 373 nm，带隙3.32 eV）。加载FA后，尺寸增加了2 nm，d间距为0.3 nm。FT-IR结果表明，FA和ZnONPS通过酰胺键连接。ZnONPS和ZnONPS @ FA的180分钟后MB的光降解分别达到82％和85％。与游离NPS相比，纳米复合材料的抗氧化活性得到增强，对粪肠球菌和其他细菌的抗菌作用得到增强。MIC和MBC值的结果分别为16至256μgmL ^-1和16至512μgmL ^{- 1}。总之，我们可以得出结论，将FA掺入NPS可以增强其抗氧化和抗菌潜能，从而使其在许多生物医学和食品包装应用中具有广阔的前景。