In vitro callus cultures of Verbena officinalis L. were maintained on solid Murashige and Skoog medium, enriched with 1 mg dm-3 BA and 1 mg dm-3 IBA under LED lights (red, blue, red/blue 70%/30%), in darkness and under control fluorescent lamps. The measurements of 2 phenylpropanoid glycosides (verbascoside and isoverbascoside) and 23 phenolic acids were performed in methanolic extracts from the biomass collected after 2-, 3- and 4-week growth cycles using the HPLC-DAD method. The presence of verbascoside, isoverbascoside and additionaly 7 phenolic acids (protocatechuic, chlorogenic, vanillic, caffeic, ferulic, o-coumaric and m-coumaric acids) was confirmed in all extracts. Blue and red/blue lights stimulated the accumulation of verbascoside (max. of 6716 and 6023 mg 100 g-1 DW after a 4-week growth cycle) and isoverbascoside (max. 333 and 379 mg 100 g-1 DW also after 4 weeks). The maximum amounts of verbascoside and isoverbascoside were respectively 1.8- and 7.0-fold higher than under the control conditions. Phenolic acids were accumulated in different amounts, and the maximum total amounts ranged from 36 to 65 mg 100 g-1 DW. LED lights also stimulated their accumulation in comparison with darkness and control. The main phenolic acids included: m-coumaric acid (max. 39 mg 100 g-1 DW), ferulic acid (max. 12 mg 100 g-1 DW), and protocatechuic acid (max. 13 mg 100 g-1 DW). Additionally, the quantities of photosynthetic pigments (chlorophyll a, b and carotenoids) were estimated in acetonic extracts using spectrophotometry. Red/blue light stimulated the biosynthesis of pigments (max. total content 287 μg g-1 FW after 4-week growth cycles). This is the first study describing the effect of LED lights on the production of phenylpropanoid glycosides and phenolic acids in V. officinalis callus cultures. Very high amounts of verbascoside and isoverbascoside are interesting from a practical point of view.

中文翻译：

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光质对体外培养马鞭草生物活性代谢产物马齿co甙，异佛手甙和酚酸的生产以及光合色素含量的影响。

马鞭草的离体愈伤组织培养物在固体Mururagege和Skoog培养基上维持，该培养基在LED灯（红色，蓝色，红色/蓝色70％/ 30％）下富含1 mg dm-3 BA和1 mg dm-3 IBA ，在黑暗中并在荧光灯的控制下。在2、3和4周的生长周期后，使用HPLC-DAD方法对从生物质中提取的甲醇提取物中的2种苯基丙烷糖苷（verbascoside和isoverbascoside）和23种酚酸进行了测量。在所有提取物中均证实了马齿bas甙，异佛手甙和另外7种酚酸（原儿茶酸，绿原酸，香草酸，咖啡酸，阿魏酸，邻香豆酸和间香豆酸）的存在。蓝色和红色/蓝色灯光刺激了马齿bas甙（在经过4周的生长后，最大值为6716和6023 mg 100 g-1 DW）和异佛手甙（最大值为）的积聚。333和379 mg 100 g-1 DW也在4周后）。马齿bas苷和异佛手苷的最大量分别比对照条件下高1.8倍和7.0倍。酚酸以不同的量积累，最大总量为36至65 mg 100 g-1 DW。与黑暗和控制相比，LED灯还刺激了它们的积累。主要酚酸包括：间香豆酸（最多39 mg 100 g-1 DW），阿魏酸（最多12 mg 100 g-1 DW）和原儿茶酸（最多13 mg 100 g-1 DW） 。此外，使用分光光度法估算了丙酮提取物中光合色素（叶绿素a，b和类胡萝卜素）的含量。红/蓝光刺激了色素的生物合成（在4周的生长周期后，最大总含量为287μgg-1 FW）。这是第一个描述LED灯对V.officinalis愈伤组织培养物中苯丙烷类糖苷和酚酸产生的影响的研究。从实用的角度来看，非常大量的马齿bas甙和异佛手甙是有趣的。