Inflammatory bowel disease (IBD) is a chronic inflammatory condition with high incidence. Syringin exhibits multiple pharmacological properties, including anti-inflammatory effects. However, the effect of syringin on inflammation of IBD is still unclear. Here, the dextran sulfate sodium (DSS)-induced colitis model was established in vivo. Rat intestinal epithelium IEC6 cells were treated with lipopolysaccharide (LPS) in vitro. Syringin inhibited DSS or LPS-induced overproduction of proinflammatory cytokines (IL-1β, IL-6, TNF-α) and proinflammatory substances (iNOS, COX-2). Moreover, syringin inactivated the proinflammatory NF-κB p65 pathway by decreasing IκBα phosphorylation at Ser 32. The activation of antioxidant Nrf2 signaling pathway was promoted by syringin. Additionally, LPS-induced inflammation in IEC6 cells was also suppressed by NF-κB inhibitor PDTC and Nrf2 activator RTA408. The anti-inflammatory effects of syringin were comparable to these two reagents. Taken together, our results suggest that syringin shows protective effects on intestinal inflammation through inhibiting NF-κB, while activating Nrf2 signaling pathway in colitis.

中文翻译：

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丁香精油可减轻炎症，从而预防结肠炎。

炎性肠病（IBD）是一种高发的慢性炎性疾病。丁香脂素具有多种药理特性，包括抗炎作用。然而，丁香脂素对IBD炎症的作用仍不清楚。在这里，体内建立了葡聚糖硫酸钠（DSS）诱导的结肠炎模型。在体外用脂多糖（LPS）处理大鼠肠上皮IEC6细胞。丁香脂素抑制DSS或LPS诱导的促炎细胞因子（IL-1β，IL-6，TNF-α）和促炎物质（iNOS，COX-2）的过量产生。此外，丁香香精素通过降低Ser 32处的IκBα磷酸化而使促炎性NF-κBp65途径失活。丁香香素促进抗氧化剂Nrf2信号通路的激活。此外，NF-κB抑制剂PDTC和Nrf2激活剂RTA408也抑制LPS诱导的IEC6细胞炎症。丁香脂素的抗炎作用与这两种试剂相当。两者合计，我们的结果表明丁香香精素通过抑制NF-κB，同时激活结肠炎中的Nrf2信号传导途径，对肠道炎症表现出保护作用。