BACKGROUND PAX8 was not only a mitotic factor, but identified as a transcription factor involved in the prognosis of human tumor patients. Elucidating the function of PAX8 on the pathology of stomach cancer was meaningful. RESULTS PAX8 was found to be upregulated in primary stomach cancer tissue and the TCGA stomach cancer dataset. Interestingly, SOX13 and PAX8 showed consistent expression patterns, and the combined high PAX8 and SOX18 expression induced a worse prognosis of stomach cancer patients. SOX13 was further identified as a transcription factor of PAX8, and further affect Aurora B and Cyclin B1 expression, two cell cycle related factors of the downstream of PAX8, including. Furthermore, PAX8 depletion inducted G1-phase arrest and the decrease of EdU incorporation, cell viability and colony formation can be rescued by SOX13 overexpression. CONCLUSIONS SOX13 participated in the elevated expression of PAX8, which promote the proliferation of stomach cancer cells. Therefore, SOX13 mediated PAX8 expression was recognized as a tumor-promoting role in stomach cancer.

中文翻译：

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PAX8表达的评估促进胃癌细胞的增殖。

背景技术PAX8不仅是有丝分裂因子，而且被鉴定为参与人类肿瘤患者预后的转录因子。阐明PAX8在胃癌病理学中的功能是有意义的。结果发现PAX8在原发性胃癌组织和TCGA胃癌数据集中被上调。有趣的是，SOX13和PAX8表现出一致的表达模式，并且PAX8和SOX18的高表达组合导致胃癌患者的预后更差。SOX13被进一步鉴定为PAX8的转录因子，并进一步影响Aurora B和Cyclin B1的表达，这是PAX8下游的两个细胞周期相关因子，包括。此外，可以通过SOX13过表达来挽救PAX8耗竭引起的G1期停滞，并降低EdU掺入，细胞活力和集落形成的减少。结论SOX13参与了PAX8的高表达，促进了胃癌细胞的增殖。因此，SOX13介导的PAX8表达被认为是胃癌的促肿瘤作用。