The aim of this work is to develop an efficient and economical method for the enrichment of total flavonoids from Pteris ensiformis Burm. extracts. Resin screening, adsorption kinetics, adsorption isotherms and thermodynamics were successively researched prior to the dynamic adsorption and desorption tests. NKA-II resin was chosen as the best adsorbent, and the adsorption data were best described by the pseudo-second-order kinetics model and Langmuir isotherm model. The optimum enrichment conditions were as follows: for adsorption the total flavonoids concentration, flow rate and volume of sample were 1.84 mg/mL, 2 BV/h and 5 BV, respectively, and for desorption the flavonoids-loaded NKA-II resin column was desorbed by 7 BV of 50% ethanol at a rate of 2 BV/h. The product had a 6.63-fold higher total flavonoids content than crude extracts, and the recovery yield of total flavonoids was 80.65%. Furthermore, flavonoids-enriched extracts exhibited higher in vitro scavenging activity against superoxide anion radical and hydroxyl radical than crude extracts. In addition, higher antiproliferative activity of flavonoids-enriched extracts against MCF-7 and HepG-2 cell lines was also found as compared to the crude extracts. The developed method is appropriate for large-scale enrichment of total flavonoids from Pteris ensiformis Burm. extracts in the food and pharmaceutical industries.

这项工作的目的是开发一种有效和经济的方法，以富集来自凤尾蕨的总黄酮。伯姆 提取物。在动态吸附和解吸测试之前，先后研究了树脂的筛选，吸附动力学，吸附等温线和热力学。选择了NKA-II树脂作为最佳吸附剂，并通过拟二级动力学模型和Langmuir等温线模型对吸附数据进行了最好的描述。最佳富集条件如下：吸附总黄酮浓度，流速和样品体积分别为1.84 mg / mL，2 BV / h和5 BV，而用于解吸黄酮负载的NKA-II树脂柱为7 BV的50％乙醇以2 BV / h的速率脱附。该产品的总黄酮含量比粗提取物高6.63倍，总黄酮的回收率为80.65％。此外，富含类黄酮的提取物表现出更高的含量。体外对超氧阴离子自由基和羟基自由基的清除能力优于粗提物。另外，与粗提取物相比，还发现了富含类黄酮的提取物对MCF-7和HepG-2细胞系的更高的抗增殖活性。所开发的方法适用于大规模浓缩凤尾蕨中总黄酮的方法。食品和制药行业中的提取物。