Although the interplay of covalent histone acetylation/deacetylation and ATP-dependent chromatin remodelling is crucial for the regulation of chromatin structure and gene expression in eukaryotes, the underlying molecular mechanism in plants remains largely unclear. Here we show a direct interaction between Arabidopsis SWI3B, an essential subunit of the SWI/SNF chromatin-remodelling complex, and the RPD3/HDA1-type histone deacetylase HDA6 both in vitro and in vivo. Furthermore, SWI3B and HDA6 co-repress the transcription of a subset of transposons. Both SWI3B and HDA6 maintain transposon silencing by decreasing histone H3 lysine 9 acetylation, but increasing histone H3 lysine 9 di-methylation, DNA methylation and nucleosome occupancy. Our findings reveal that SWI3B and HDA6 may act in the same co-repressor complex to maintain transposon silencing in Arabidopsis.

中文翻译：

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SWI3B和HDA6相互作用，是拟南芥转座子沉默所必需的。

尽管共价组蛋白乙酰化/去乙酰化和ATP依赖的染色质重塑之间的相互作用对于调节真核生物中的染色质结构和基因表达至关重要，但植物中的潜在分子机制仍不清楚。在这里，我们显示了拟南芥SWI3B（SWI / SNF染色质重塑复合体的重要亚基）与RPD3 / HDA1型组蛋白脱乙酰基酶HDA6之间的直接相互作用。此外，SWI3B和HDA6共同抑制转座子子集的转录。SWI3B和HDA6都通过降低组蛋白H3赖氨酸9的乙酰化，但增加组蛋白H3赖氨酸9的二甲基化，DNA甲基化和核小体占用来维持转座子沉默。